Back to results
US 2002/0137039 A1Published application
5' Nuclease nucleic acid amplification assay having an improved internal control
AssigneeBaxter Aktiengesellschaft
InventorMatthias Gessner
Life of this patent
Estimating the term…
Abstract
Nucleic acid amplification assays using a 5′ nuclease and having internal amplification controls are provided. Related methods for preparing the internal controls are also provided. Moreover, methods for rapidly and accurately determining optimum nucleic acid sequences for the internal amplification controls the 5′ nuclease assays are provided.
Claims20
A method for preparing an internal control for a 5′ nuclease polymerase chain reaction (PCR) assay comprising:
- determining a nucleic acid sequence for a target oligonucleotide probe binding site;
- inverting said nucleic acid sequence of said target oligonucleotide probe binding site;
- constructing an internal control oligonucleotide having said inverted target oligonucleotide probe binding site integrated therein;
- constructing an internal control probe having a nucleic acid sequence complementary to said inverted target oligonucleotide probe binding site, wherein said internal control probe hybridizes with said inverted target nucleic acid probe binding site sequence integrated into said internal oligonucleotide control but not with said nucleic acid sequence of said target oligonucleotide probe binding site.
1 more element
A 5′ nuclease PCR assay having an internal control wherein said internal control comprises:
- an oligonucleotide having at least part of its nucleic acid sequence an inverse of a target oligonucleotide probe binding site nucleic acid sequence;
- an internal control probe having a nucleic acid sequence complementary to said inverted target oligonucleotide probe binding site, wherein said internal control probe hybridizes with said inverted target nucleic acid probe binding site sequence but not with said target oligonucleotide probe binding site nucleic acid sequence.
An HCV 5′ nuclease PCR assay comprising:
- a first probe having a first detectable label, said first probe having a nucleic acid sequence complementary to a target HCV oligonucleotide probe binding sequence;
- a 5′ nuclease enzyme;
- a second probe having a second detectable label, said second probe having a nucleic acid sequence complementary to an internal standard oligonucleotide probe binding sequence, said internal standar oligonucleotide probe binding sequence being the inverse of said target HCV oligonucle tide probe binding sequence;
- at least one primer complementary to primer binding sites on said target HCV nucleotide and said internal standard oligonucleotide.
- at least one primer complementary to primer binding sites on said target HCV nucleotide
2 more elements
Dependencies are inferred from the claim text.