Glucuronyl C5-epimerase, DNA encoding the same and uses thereof
AssigneeBiotie Therapies, Inc.
InventorsMarkku Jalkanen, Kamel El Darwish, Ulf Lindahl, Jin-Ping Li
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Abstract
The invention is directed to a novel purified mouse C5-epimerase, fragments thereof, nucleic acids encoding the same and the recombinant production thereof. The invention is also directed to fragments of such epimerase, especially N-terminal fragments that are useful in fusion protein constructs to enhance the activity of recombinantly-produced heterologous epimerase enzymes.
Claims18
An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide, the amino acid sequence of which is at least 95% identical to a reference amino acid sequence selected from the group consisting of:
- (a) amino acids 1 to 118 of FIG. 3;
- (b) amino acids 1 to 119 of FIG. 3;
- (c) amino acids 1 to 120 of FIG. 3;
- (d) amino acids 1 to 121 of FIG. 3;
- (e) amino acids 119 to 618 of FIG. 3;
- (f) amino acids 120 to 618 of FIG. 3;
- (g) amino acids 121 to 618 of FIG. 3;
- (h) amino acids 122 to 618 of FIG. 3;
- (i) amino acids 34 to 147 of FIG. 3;
- (j) amino acids 35 to 154 of FIG. 3;
- (k) amino acids 34 to 154 of FIG. 3;
- (l) amino acids 1 to 154 of FIG. 3;
- (m) amino acids 155-618 of FIG. 3 and
- (n) amino acids 1-618 of FIG. 3.
An isolated C5-epimerase polypeptide, the amino acid sequence of which is at least 95% identical to a sequence selected from the group consisting of:
- (a) amino acids 1 to 118 of FIG. 3;
- (b) amino acids 1 to 119 of FIG. 3;
- (c) amino acids 1 to 120 of FIG. 3;
- (d) amino acids 1 to 121 of FIG. 3;
- (e) amino acids 119 to 618 of FIG. 3;
- (f) amino acids 120 to 618 of FIG. 3;
- (g) amino acids 121 to 618 of FIG. 3;
- (h) amino acids 122 to 618 of FIG. 3;
- (i) amino acids 34 to 147 of FIG. 3;
- (j) amino acids 35 to 154 of FIG. 3;
- (k) amino acids 34 to 154 of FIG. 3;
- (l) amino acids 1 to 154 of FIG. 3;
- (m) amino acids 155-618 of FIG. 3;
- and
- (n) amino acids 1-618 of FIG. 3.
A method of increasing the activity of a C5-epimerase, said method comprising:
- (a) providing a first polynucleotide comprising a nucleotide sequence encoding a polypeptide, the amino acid sequence of which is at least 80% identical to a reference amino acid sequence selected from the group consisting of amino acids 35 to 154 of FIG. 3 and amino acids 34 to 154 of FIG. 3;
- (b) attaching said first polynucleotide of (a) to a second polynucleotide encoding a C5-epimerase;
- and
- (c) expressing the fusion polynucleotide.
Dependencies are inferred from the claim text.