Genetic control of fruit ripening
OwnerColin Roger Bird, Graham Barron Seymour +1inventors
InventorsColin Roger Bird, Graham Barron Seymour, Rosybel De Jesus Medina Suarez
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Abstract
A method of modulating the ripening and/or senescence characteristics in plants of the genus Musa comprises transforming plants with one or more sequences obtainable from the deposited cDNA library having the accession number 40184, regenerating said plants and selecting from the population of transformants those plants having modulated and/or tissue senescence characteristics.
Claims15
A method of modulating the ripening or tissue senescence process in plants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence selected from the sequences depicted as SEQ ID Nos 1-57, regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics.
A method of modulating the ripening or tissue senescence process in plants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence or a fragment thereof, obtainable by hybridisation, from the cDNA library having the NCIMB Accession Number 40814, by the use of at least one of the sequences depicted as SEQ ID Nos 1-57 as oligonucleotide probes, said hybridisation being conducted at a temperature from 60° C. to 65° C. in 0.3 strength citrate buffered saline containing 0.1% SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1% SDS, regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics.
A method according to any preceding claim wherein the plant material is transformed using the Agrobacterium, microparticle bombardment, fibre mediated or direct insertion method.
A polynucleotide associated with fruit ripening selected from SEQ ID Nos. 1-57.
A method, polynucleotide, plant, its progeny, seed and material obtained from said plants and a banana substantially as hereinbefore described with reference to the accompanying drawings and figures.
Dependencies are inferred from the claim text.