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AssigneeKorea Kumho Petrochemical Co., Ltd.
InventorsGiltsu Choi, Eric I. Johnson, Hankuil Yi, Byongchul Shin
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The invention includes modified dihydroflavonol 4-reductase (DFR) nucleic acids encoding the modified DFR that has altered amino acid sequences at the substrate specificity determining region. The property of the modified DFR is characterized by its ability to reduce dihydrokaempferol (DHK) preferentially over dihydroquercetin (DHQ), and dihydromyricetin (DHM). The invention also includes plants having at least one cell expressing the modified DFR. Such plants are characterized by the increased content of pelargonidin-based pigments. The invention also includes vectors comprising at least a portion of the modified DFR nucleic acids. The invention also includes methods using such vectors for producing plants having the increased content of pelargonidin-based pigments.
The substrate specific DFR nucleic acids and encoding DFRs that have altered amino acid sequences at the substrate specificity determining region due to the altered nucleic acid sequences encoding amino acids at the substrate specificity determining region.
A vector capable of transforming a plant cell to increase the content of one class of pigments preferentially in a plant containing said cell, said vector comprising at least a portion of the substrate specific DFR nucleic acids operably linked to a promoter.
A method for producing a plant having a phenotype characterized by the increased content of one class of pigments, said method comprising at least a step of:
Dependencies are inferred from the claim text.