In vitro synthesis of a layered cell sorted tissue
AssigneeXgene Corporation
InventorsWarren K. Hoeffler, Deborah Finlay
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Abstract
The present invention relates to a layered cell sorted tissue that is formed in vitro. The tissue is generated by the spontaneous sorting of cells from a homogenous cell mixture into discrete layers by cell type. Connective tissue components, such as fibronectin, may be used to manipulate orientation of the layers during the cell sorting process. The layered cell sorted tissue may be used as a skin graft for bums, wounds, and ulcers. The tissue may also be used in assays to determine effects of chemicals or drugs on human tissue in vitro as well as provide an in vitro assay for tumor cell metastasis.
Claims37
A layered cell sorted tissue comprising a discrete first cell layer and a discrete second cell layer, wherein the cells spontaneously sort from a homogenous cell mixture to form said discrete first and second layers, and wherein said tissue is formed in vitro.
An in vitro method for generating a layered cell sorted tissue comprising a discrete first cell layer and a discrete second cell layer comprising the steps of:
- providing a homogenous mixture of cells;
- contacting said mixture with a connective tissue component;
- and
- allowing said mixture to spontaneously sort into said discrete layers.
A method for grafting skin comprising the steps of:
- providing a homogenous mixture of keratinocytes and fibroblasts;
- applying the mixture to a graft site;
- and
- allowing the mixture to spontaneously sort into a discrete layer comprising keratinocytes and a discrete layer comprising fibroblasts.
A composition of cells for making a layered cell sorted tissue in vitro comprising a homogenous mixture of fibroblasts and a second cell type, and 10% fetal bovine serum.
Dependencies are inferred from the claim text.