Plant protein kinases
AssigneeE. I. Du Pont de Nemours and Company
InventorStephen M. Allen
Life of this patent
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a protein kinase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the protein kinase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the protein kinase in a transformed host cell.
Claims30
An isolated nucleic acid fragment encoding a calcium dependent phosphorylase kinase comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding an amino acid sequence of at least 100 contigous amino acids that is at least 90% identical to the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, 6 and 8;
- (b) an isolated nucleic acid fragment that is complementary to (a).
A calcium dependent phosphorylase kinase polypeptide comprising the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, 6 and 8.
An isolated nucleic acid fragment encoding a calcium dependent phosphorylase kinase comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding the amino acid sequence set forth in SEQ ID NO:4;
- (b) an isolated nucleic acid fragment that is complementary to (a).
A calcium dependent phosphorylase kinase polypeptide comprising the amino acid sequence set forth in SEQ ID NO:4.
An isolated nucleic acid fragment encoding a glycogen synthase kinase comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding an amino acid sequence of at least 400 amino acids that is at least 90% identical to the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:
- 10 and 16;
- (b) an isolated nucleic acid fragment that is complementary to (a).
A glycogen synthase kinase polypeptide comprising all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:10 and 16.
An isolated nucleic acid fragment encoding a glycogen synthase kinase comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding an amino acid sequence of at least 105 contigous amino acids that is at least 80% identical to the amino acid sequence set forth in SEQ ID NO:
- 12;
- (b) an isolated nucleic acid fragment that is complementary to (a).
A glycogen synthase kinase polypeptide comprising the amino acid sequence set forth in SEQ ID NO:12.
An isolated nucleic acid fragment encoding a glycogen synthase kinase comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding the amino acid sequence set forth in SEQ ID NO:14;
- (b) an isolated nucleic acid fragment that is complementary to (a).
A glycogen synthase kinase polypeptide comprising the amino acid sequence set forth in SEQ ID NO:14.
Dependencies are inferred from the claim text.