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AssigneeMETABION Gesellschaft fur angewandte Biotechnologie mbH
InventorLudwig Stockl
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In a process and a device for the parallel preparation of at least 4n oligonucleotides, at least four inserts each with n reaction vessels are first arranged on a plate (16), each reaction vessel containing a nucleotide initiator base bound to an inert carrier. Particular operations are then carried out in parallel with one another at four stations (28, 30, 32, 34), and in particular a deblocking operation simultaneously in all n reaction vessels of the insert at the first station (28), a first washing operation simultaneously in all n reaction vessels of the insert at the second station (30), a coupling operation in all n reaction vessels of the insert at the third station (32), and, simultaneously in all n reaction vessels of the insert at the fourth station (34), a second washing operation followed by a capping operation followed by a third washing operation followed by an oxidation operation followed by a fourth washing operation. The plate (16) with the inserts is rotated station by station, the abovementioned operations being carried out, until the desired oligonucleotides have been formed by coupling individual nucleotides to one another.
Process for the parallel preparation of at least 4n oligonucleotides, with the steps:
Process according to one of the preceding claims, characterized in that in the coupling operation carried out at the third station, a selected nucleotide base is added to the reaction vessels simultaneously with an activator, preferably tetrazole.
Process according to one of the preceding claims, characterized in that in the coupling operation carried out at the third station, a marking group, in particular a base analogue, a dyestuff or a hapten, is added to the reaction vessels.
Process according to one of the preceding claims, characterized in that the reaction vessels are constructed as flow-through vessels, and in that the liquids to be fed to the reaction vessels in the four stations are conveyed into the reactions vessels and out of them by applying a pressure gradient between the reaction vessel inlet and reaction vessel outlet.
Process according to one of the preceding claims, characterized in that all the operations are carried out under an inert gas atmosphere, in particular under nitrogen and/or argon.
Device for the parallel preparation of at least 4n oligonucleotides, comprising
Dependencies are inferred from the claim text.