Nucleic acid sequences of genes encoding high mobility group proteins and uses thereof
OwnerJorn Bullerdiekinventor
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Abstract
The invention relates to DNA-sequences, their use and the use of DNA-sequences of the MAG gene or genes encoding the high mobility group proteins, agents for the treatment of various diseases including tumors, influencing the development of the vascular system, as well as for contraception and tissue regeneration, and appropriate kits and processes. The sequences, agents, uses, kits and processes enable the specific influencing of molecular mechanisms that jointly form the basis for various diseases, the development of the vascular system, the contraception and the regeneration of tissue. Thus, the disadvantages associated with other agents or processes are decreased.
Claims68
An isolated polynucleotide having a sequence comprising:
- a first sequence encoding each of exons 1, 2 and 3 of an HMGI or MAG gene;
- and
- a second sequence from a source other than an HMGI or MAG gene, said second sequence being from a human and being located upstream of said exons, said sequence also being a sequence other than a sequence immediately upstream of exon 1 of said HMGI or MAG gene in a human genome.
An isolated polypeptide having a sequence comprising:
- a first sequence which is encoded by exons 1, 2 and 3 of an HMGI or MAG gene;
- and
- a second sequence upstream of said first sequence, said sequence being encoded by a polynucleotide from a source other than an HMGI or MAG gene, said polynucleotide being from a human, said polynucleotide having a sequence other than a sequence immediately upstream of exon 1 of said HMGI or MAG gene in a human genome.
A method for treatment of tumors in a mammal, comprising administering to said mammal an agent which blocks the activity or effect of a DNA selected from the group consisting of an HMGI gene, a MAG gene and a polynucleotide having a sequence comprising:
- a first sequence encoding each of exons 1, 2 and 3 of an HMGI or MAG gene;
- and
- a second sequence from a source other than an HMGI or MAG gene, said second sequence being from a human and being located upstream of said exons, said sequence also being a sequence other than a sequence immediately upstream of exon 1 of said HMGI or MAG gene in a human genome, or which blocks the effect or activity of a transcription or translation product of said DNA.
A method for diagnosis of tumors, comprising:
- administering to suspected tumor tissue a first polyncleotide complementary to a second polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- detecting significant complex formation between said first polynucleotide and said second polynucleotide when a tumor is present, but not when a tumor is absent.
A method for diagnosis of tumors, comprising:
- administering to suspected tumor tissue a translation product of a polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- detecting significant complex formation between said translation product and said polynucleotide when a tumor is present, but not when a tumor is absent.
A method for diagnosis of tumors, comprising:
- administering to suspected tumor tissue an antibody to a translation product of a polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- detecting significant complex formation between said antibodies and said translation products when a tumor is present, but not when a tumor is absent.
A method for diagnosis of endometriosis, comprising:
- administering a first polyncleotide to suspected ectopic endometrial tissue complementary to a second polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- detecting significant complex formation between said first polynucleotide and said second polynucleotide when endometriosis is present, but not when endometriosis is absent.
A method for diagnosis of endometriosis, comprising:
- administering to suspected ectopic endometrial tissue a translation product of a polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- detecting significant complex formation between said translation product and said polynucleotide when endometriosis is present, but not when endometriosis is absent.
A method for diagnosis of endometriosis, comprising:
- administering to suspected ectopic endometrial tissue an antibody to a translation product of a polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- detecting significant complex formation between said antibodies and said translation products when endometriosis is present, but not when endometriosis is absent.
A method for contraception in a mammal, comprising:
- administering to said mammal a first polyncleotide complementary to a second polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- forming a complex between said first polynucleotide and said second polynucleotide, thereby inhibiting fertility.
A method for contraception in a mammal, comprising:
- administering to said mammal a translation product of a polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- forming a complex between said translation product and said polynucleotide thereby inhibiting fertility.
A method for contraception in a mammal, comprising:
- administering to said mammal an antibody to a translation product of a polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- forming a complex between said antibodies and said translation products, thereby inhibiting fertility.
A method for regenerating tissue, comprising activating expression in said tissue of a nucleic acid sequence selected from the group consisting of HMGI genes, MAG genes, nucleic acid sequences according to SEQ ID Nos. 1-19 and their derivatives in a cell.
A method according to claim 46, wherein the mesenchymal tissue is selected from the group comprising cartilage tissue, muscle tissue, fatty or adipose tissue, connective tissue and supporting tissue.
A method for regenerating tissue, comprising administering to said tissue a translation product of a nucleic acid sequence selected from the group consisting of HMGI genes, MAG genes, nucleic acid sequences according to SEQ ID Nos. 1-19 and their derivatives.
A method for inducing angiogenesis, comprising activating expression in venous or capillary tissue of a nucleic acid sequence selected from the group consisting of HMGI genes, MAG genes, nucleic acid sequences according to SEQ ID Nos. 1-19 and their derivatives in a cell.
A method for inducing angiogensis, comprising administering to venous or capillary tissue a translation product of a nucleic acid sequence selected from the group consisting of HMGI genes, MAG genes, nucleic acid sequences according to SEQ ID Nos. 1-19 and their derivatives.
A method for inhibiting angiogenesis in a mammal, comprising:
- administering to said mammal a first polyncleotide complementary to a second polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- forming a complex between said first polynucleotide and said second polynucleotide, thereby inhibiting angiogenesis.
A method for inhibiting angiogenesis in a mammal, comprising:
- administering to said mammal a translation product of a polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- forming a complex between said translation product and said polynucleotide thereby inhibiting angiogenesis.
A method for inhibiting angiogenesis in a mammal, comprising:
- administering to said mammal an antibody to a translation product of a polyncleotide selected from the group consisting of HMGI genes, MAG genes and nucleic acid molecules having sequences according to one of SEQ ID NOS.:
- 1-19;
- and
- forming a complex between said antibodies and said translation products, thereby inhibiting angiogenesis.
Dependencies are inferred from the claim text.