2,5-DKG permeases
AssigneeGenencor International, Inc.
InventorsVeronique A. Dartois, James A. Hoch, Fernando Valle, Manoj Kumar
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Abstract
The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease activity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.
Claims48
An isolated nucleic acid molecule encoding a polypeptide which has 2,5-DKG permease activity.
An isolated oligonucleotide, comprising at least 20 contiguous nucleotides of a nucleotide sequence selected from the group consisting of SEQ ID NOS:1, 3, 5, 7 and 9.
An isolated oligonucleotide, comprising a nucleotide sequence encoding a peptide having at least 10 contiguous amino acids of an amino acid sequence selected from the group consisting of SEQ ID NOS:2, 4, 6, 8 and 10.
An isolated polypeptide which has 2,5-DKG permease activity.
An isolated peptide, comprising at least 10 contiguous amino acids of any of SEQ ID NOS:2, 4, 6, 8 and 10, wherein said peptide is immunogenic.
Dependencies are inferred from the claim text.