Enterococcus faecalis polynucleotides and polypeptides
OwnerCharles A. Kunsch, Patrick J. Dillon +1inventors
InventorsCHARLES A. KUNSCH, PATRICK J. DILLON, STEVEN BARASH
Life of this patent
Estimating the term…
Abstract
The present invention provides polynucleotide sequences of the genome of Enterococcus faecalis, polypeptide sequences encoded by the polynucleotide sequences, corresponding polynucleotides and polypeptides, vectors and hosts comprising the polynucleotides, and assays and other uses thereof. The present invention further provides polynucleotide and polypeptide sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use.
Claims18
Computer readable medium having recorded thereon the nucleotide sequence depicted in SEQ ID NOS:
- 1-982, a representative fragment thereof or a nucleotide sequence at least 95% identical to a nucleotide sequence depicted in SEQ ID NOS:1-982.
A computer-based system for identifying fragments of the Enterococcus faecalis genome of commercial importance comprising the following elements:
- a) a data storage means comprising the nucleotide sequence of SEQ ID NOS:1-982, a representative fragment thereof, or a nucleotide sequence at least 95% identical to a nucleotide sequence of SEQ ID NOS:1-982;
- b) search means for comparing a target sequence to the nucleotide sequence of the data storage means of step (a) to identify homologous sequence(s), and
- c) retrieval means for obtaining said homologous sequence(s) of step (b).
A method for identifying commercially important nucleic acid fragments of the Enterococcus faecalis genome comprising the step of comparing a database comprising the nucleotide sequences depicted in SEQ ID NOS:1-982, a representative fragment thereof, or a nucleotide sequence at least 95% identical to a nucleotide sequence of SEQ ID NOS:1-982 with a target sequence to obtain a nucleic acid molecule comprised of a complementary nucleotide sequence to said target sequence, wherein said target sequence is not randomly selected.
A method for identifying an expression modulating fragment of Enterococcus faecalis genome comprising the step of comparing a database comprising the nucleotide sequences depicted in SEQ ID NOS:
- 1-982, a representative fragment thereof, or a nucleotide sequence at least 95% identical to the nucleotide sequence of SEQ ID NOS:1-982 with a target sequence to obtain a nucleic acid molecule comprised of a complementary nucleotide sequence to said target sequence, wherein said target sequence comprises sequences known to regulate gene expression.
An isolated protein-encoding nucleic acid fragment of the Enterococcus faecalis genome, wherein said fragment consists of the nucleotide sequence of any one of the fragments of SEQ ID NOS:1-982 depicted in Tables 2 and 3, or a degenerate variant thereof.
Dependencies are inferred from the claim text.