Pesticidal toxins and genes from bacillus laterosporus strains
AssigneeMycogen Corporation
InventorsH. Ernest Schnepf, Kenneth E. Narva, Brian A. Stockhoff, Stacey Finstad Lee, Mikki Walz, Blake Sturgis
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Abstract
Disclosed and claimed are novel toxins and genes obtainable from Bacillus laterosporus isolates disclosed herein. In preferred embodiments, the subject genes and toxins are used to control Western corn rootworm.
Claims18
An isolated protein that has toxin activity against a corn rootworm pest, wherein said protein comprises
- a. an amino acid that has at least 90% identity with SEQ ID NO:9 and retains the toxin activity of the polypeptide defined by SEQ ID NO:9;
- b. the amino acid sequence of SEQ ID NO:9 or a segment thereof that retains the toxin activity of the polypeptide defined by SEQ ID NO:9;
- c. an amino acid sequence that has at least 90% identity with SEQ ID NO:7 and retains the toxin activity of the polypeptide defined by SEQ ID NO:7;
- or
- d. the amino acid sequence of SEQ ID NO:7 or a segment thereof that retains the toxin activity of the polypeptide defined by SEQ ID NO:7.
An isolated protein that has toxin activity against a corn rootworm pest wherein said protein is obtainable from Bacillus laterosporus isolate MB439 having accession number NRRL B-20086, said isolate comprising a DNA segment that encodes said toxin, wherein said DNA segment is selected from the group consisting of a DNA segment that comprises the nucleotide sequence of SEQ ID NO:10, and a DNA segment that hybridizes with the nucleic acid sequence of SEQ ID NO:2 when said nucleic acid sequence is used as a probe.
An isolated polynucleotide that encodes a protein that has toxin activity against a corn rootworm pest, wherein the full complement a nucleotide sequence that encodes the polypeptide of SEQ ID NO:9 hybridizes with said polynucleotide when said complement is used as a probe, wherein hybridization is maintained at conditions of 0.1× SSPE at 65° C.
An isolated polynucleotide that encodes a protein that has toxin activity against a corn rootworm pest wherein said protein is obtainable from E. coli clone MR957 having accession number NRRL B-30048, said clone comprising a DNA segment encoding said toxin, wherein the nucleotide sequence of SEQ ID NO:2, when used as a probe, hybridizes with said DNA segment.
An isolated protein having a molecular weight of approximately 1-10 kDa wherein said protein has toxin activity against a corn rootworm pest, and wherein said protein is obtainable from a Bacillus laterosporus isolate selected from the group consisting of MB438 having accession number NRRL B-30085 and MB439 having accession number NRRL B-20086.
A method of controlling a corn rootworm pest wherein said method comprises contacting said pest with an isolated protein, wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 7 and SEQ ID NO:9.
Dependencies are inferred from the claim text.