Biologically active proteins
OwnerGennady P. Samokhin, Vladimir P. Torchilin +2inventors
InventorsGennady P. Samokhin, Vladimir P. Torchilin, Leonid Z. Iakoubov, Dmitriy A. Mongayt
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Abstract
Methods are disclosed for preparing biologically active protein conjugates using negatively charged polymers that protect the protein within the conjugate so that it retains a substantial amount of its biological activity following conjugation (e.g., procedure in which a protein is conjugated to a pharmaceutical agent, a solid support, a reporter molecule, a group carrying a reporter molecule, an acylating agent, a chelating agent, a cross-linking agent, a targeting group, and a ligand and binding group). The invention also includes novel protein conjugates prepared by those methods.
Claims43
A method of preparing a biologically active protein conjugate, the method comprising:
- combining a biologically active protein moiety with a protective group under conditions that allow the protective group to removably bind to the protein to provide a protected protein;
- modifying the protected protein by the addition of a modifying agent moiety selected from the group consisting of a pharmaceutical agent, a solid support, a reporter group, and a targeting group;
- and
- removing the protective group to provide a biologically active protein conjugate comprising a biologically active protein moiety and the modifying agent moiety.
A biologically active protein conjugate comprising a first moiety and a second moiety, wherein the first moiety is a biologically active protein and wherein the second moiety is selected from the group consisting of a pharmaceutical agent, a solid support, a reporter molecule, and a targeting group.
A method of preparing a biologically active protein conjugate, the method comprising:
- combining a biologically active protein with a protective group selected from the group consisting of anionic polysaccharides, anionic oligosaccharides, and mixtures thereof to provide a protected protein under conditions that allow the protective group to removably bind to the protein;
- and
- modifying the protected protein by the addition of a modifying agent selected from the group consisting of a pharmaceutical agent, a solid support, a reporter molecule, a group carrying a reporter molecule, a chelating agent, an acylating agent, a cross-linking agent, and a targeting group.
A biologically active protein conjugate comprising a first moiety and a second moiety, the first moiety is a protected biologically active protein and the second moiety being selected from the group consisting of a pharmaceutical agent, a solid support, a reporter molecule, a group carrying a reporter molecule, a chelating agent, an acylating agent, a cross-linking agent, and a targeting group, the protected biologically active protein being associated with a protective group selected from the group consisting of anionic polysaccharides, anionic oligosaccharides and mixtures thereof.
Dependencies are inferred from the claim text.