Monoclonal antibody recognizing phosphatidylinositol-3,4,5-triphosphate
AssigneeMedical & Biological Laboratories Co., Ltd.
InventorsYasuhisa Fukui, Satoshi Nagata, Ryuichi Shirai
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Abstract
A novel monoclonal antibody that specifically recognizes phosphatidylinositol-3,4,5-triphosphate (PIP3) but does not cross-react with structurally similar phospholipid antigens is advantageous for PIP3-specific immunoassay. The gene in the variable regions of the monoclonal antibody has been identified, which enables producing recombinant antibodies.
Claims17
An antibody specifically recognizing phosphatidylinositol-3,4,5-triphosphate.
A variable region of immunoglobulin heavy chain specifically binding to phosphatidylinositol-3,4,5-triphosphate, comprising an amino acid sequence shown by SEQ ID NO:
- 2 or an amino acid sequence of SEQ ID NO:
- 2 in which one or more amino acid residues have been substituted, deleted or added.
A variable region of immunoglobulin light chain specifically binding to phosphatidylinositol-3,4,5-triphosphate, comprising an amino acid sequence shown by SEQ ID NO:
- 4 or an amino acid sequence of SEQ ID NO:
- 4 in which one or more amino acid residues have been substituted, deleted or added.
CDR1 in immunoglobulin heavy chains specifically binding to phosphatidylinositol-3,4,5-triphosphate, comprising an amino acid sequence shown by SEQ ID NO:
- 5 or an amino acid sequence of SEQ ID NO:5 in which one or more amino acid residues have been substituted, deleted or added.
CDR2 in immunoglobulin heavy chains specifically binding to phosphatidylinositol-3,4,5-triphosphate, comprising an amino acid sequence shown by SEQ ID NO:6 or an amino acid sequence of SEQ ID NO:6 in which one or more amino acid residues have been substituted, deleted or added.
CDR3 in immunoglobulin heavy chains specifically binding to phosphatidylinositol-3,4,5-triphosphate, comprising an amino acid sequence shown by SEQ ID NO:7 or an amino acid sequence of SEQ ID NO:7 in which one or more amino acid residues have been substituted, deleted or added.
CDR1 in immunoglobulin light chains specifically binding to phosphatidylinositol-3,4,5-triphosphate, comprising an amino acid sequence shown by SEQ ID NO:8 or an amino acid sequence of SEQ ID NO:8 in which one or more amino acid residues have been substituted, deleted or added.
CDR2 in immunoglobulin light chains specifically binding to phosphatidylinositol-3,4,5-triphosphate, comprising an amino acid sequence shown by SEQ ID NO:9 or an amino acid sequence of SEQ ID NO:9 in which one or more amino acid residues have been substituted, deleted or added.
CDR3 in immunoglobulin light chains specifically binding to phosphatidylinositol-3,4,5-triphosphate, comprising an amino acid sequence shown by SEQ ID NO:10 or an amino acid sequence of SEQ ID NO:10 in which one or more amino acid residues have been substituted, deleted or added.
An immunogen composition for use in producing an antibody specifically recognizing phosphatidylinositol-3,4,5-triphosphate, comprising a mixture of a dead Salmonella cell as an adjuvant and phosphatidylinositol-3,4,5-triphosphate.
A method for producing an antibody specifically recognizing phosphatidylinositol-3,4,5-triphosphate, which comprises immunizing an immunogen composition comprising a mixture of a dead Salmonella cell as an adjuvant and phosphatidylinositol-3,4,5-triphosphate.
An immunoassay method which comprises the steps of reacting the antibody specifically recognizing phosphatidylinositol-3,4,5-triphosphate or a variable region thereof with phosphatidylinositol-3,4,5-triphosphate present in a sample, and detecting the binding based on an immunological reaction between the antibody or a variable region thereof and the triphosphate.
A kit for immunoassay for phosphatidylinositol-3,4,5-triphosphate comprising the antibody specifically recognizing phosphatidylinositol-3,4,5-triphosphate or a variable region thereof.
Dependencies are inferred from the claim text.