Intracellular delivery of biological effectors
AssigneeUniversite de Lausanne
InventorChristophe Bonny
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Abstract
The invention relates to a sequence of amino acids with the capacity to facilitate transport of an effector across a biological membrane. More specifically, the present invention relates to novel peptide transporters that specifically target certain cell types for the intracellular delivery of drugs and therapeutic agents.
Claims35
A transporter peptide comprising at least one amino acid sequence selected from the group consisting of:
- a) (XmRXoRXn);
- b) (XmRRRXn);
- c) (XmRRXRXn);
- and
- d) (XmRXRRXn),
- wherein
- X is a non-basic amino acid;
- m is an integer from zero to fourteen;
- n is an integer, independent of in, between zero and fourteen;
- o is an integer, independent of m and n between zero and five;
- and
- wherein said transporter peptide is capable of translocating across a biological membrane.
A method of translocating an effector into the cytoplasm and nucleus of a eukaryotic cell, said method comprising:
- a) conjugating said effector to the transporter peptide of claim I to form a transporter peptide-effector conjugate;
- and
- b) introducing said transporter peptide-effector conjugate to the cell.
A method of screening a phage library for transporter peptides, said method comprising:
- a) providing a phage display library;
- b) screening said library against specific cell types;
- and
- c) determining the cells having internalized phages.
A transporter peptide, wherein the amino acid sequence is selected from the group consisting of SEQ ID NOS:
- 1-34.
Dependencies are inferred from the claim text.