Signal enhancement of bispecific antibody-polymer probe for immunoassay use
OwnerBan-an Khaw, Jagat Narulainventors
Life of this patent
Estimating the term…
Abstract
An immunoassay method including reacting a sample from a patient with a bispecific antibody, wherein the bispecific antibody includes one antibody specific for a compound to be detected and a second antibody specific for a compound foreign to said patient sample, and subsequently reacting the patient sample with a polymer probe, wherein the polymer probe includes a compound recognized by the second antibody in the bispecific antibody complex and further includes at least two detectable signals; the bispecific antibody; and the polymer probe of the immunoassay method are disclosed.
Claims6
An immunoassay method comprising
- reacting a sample from a patient with a bispecific antibody, said bispecific antibody comprising
- one antibody specific for a compound to be detected and
- a second antibody specific for a compound foreign to said patient sample;
- and
- subsequently reacting said sample with a polymer probe, said polymer probe comprising
- a polymer backbone,
- attached to said polymer backbone, a compound recognizable by said second antibody in said bispecific antibody, and
- at least two detectable signal compounds further attached to said polymer backbone.
A bispecific antibody for use in an immunoassay comprising
- one antibody specific for a compound to be detected in said immunoassay;
- and
- a second antibody specific for a compound foreign to a sample to be assayed in said immunoassay.
A polymer probe comprising
- a polymer backbone;
- a compound recognizable by an antibody in a bispecific antibody attached to said polymer backbone, and
- at least two detectable signal compounds attached to said polymer backbone.
- a compound recognizable by said second antibody in said bispecific antibody and
- at least two detectable signal compounds.
Dependencies are inferred from the claim text.