Detection of analytes
OwnerGeorge Y. Daniloff, Aristotle G. Kalivretenos +2inventors
InventorsGeorge Y. Daniloff, Aristotle G. Kalivretenos, Alexandre V. Nikolaitchik, Edwin F. Ullman
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Abstract
Disclosed are methods for detecting analytes with indicator systems which may undergo a molecular configurational change upon exposure to the analyte. The configurational change affects a detectable quality associated with the indicator system, thereby allowing detection of the presence or concentration of the analyte.
Claims24
A method for detecting the presence or concentration of a polyhydroxyl analyte in a sample, which comprises:
- a) exposing the sample to an indicator system having
- i) a first recognition element capable of forming a covalent bond in a reversible fashion with said analyte, and either A) a second recognition element capable of forming a covalent bond in a reversible fashion to said analyte bound to the first recognition element, or B) a ligand element capable of interacting in a reversible fashion with the first recognition element in the absence of said analyte, said ligand element optionally further comprising a label that produces a detectable quality that is modulated by the interaction of the ligand element with the recognition element, wherein the portion of the indicator system containing said first recognition element is covalently or non-covalently linked to the portion of the indicator system containing said second recognition element or said ligand element;
- and
- ii) a detection system which comprises at least one of A) a donor/acceptor system which produces a detectable quality that changes in a concentration-dependent manner when said indicator system is exposed to said analyte, or B) said labeled ligand element;
- and
- b) measuring any change in said detectable quality to thereby determine the presence or concentration of said analyte in said sample.
An indicator system which comprises a residue of a compound selected from the group consisting of:
- N-2-[5-(N-4-dimethylaminobenzyl)-5-[2-(borono)-benzyl]aminohexyl]-[2-(borono)benzyl]aminoethyl-4-butylamino-1,8-naphthalimide;
- N-2-[4-(N-4-dimethylaminobenzyl)-[2-(borono)-benzyl]aminomethyl]benzyl-[2-(borono)benzyl]aminoethyl-4-butylamino-1,8-naphthalimide;
- N-2-[5-(N-4-dimethylaminobenzyl)-5-[2-(borono)-benzyl]aminohexyl]-[2-(borono)benzyl]aminoethyl-4-[2-(2-aminoethoxy)ethoxyethyl)amino-1,8-naphthalimide;
- N-(5-methoxycarbonyl-5-[3,4-dihydroxybenz-amido]pentyl)-N′-(5-fluoresceinyl)thiourea;
- N-α-(3-boronato-5-nitro)benzoyl-Nε-(4-dimethylamino-3,5-dinitro)benzoyllysine;
- N-α-(3,4-dihydroxybenzoyl)-N-ε-(5-dimethylaminonaphthalene-1-sulfonyl)-lysine;
- N-α-(3,4-dihydroxybenzoyl)-N-ε-(5-dimethylaminonaphthalene-1-sulfonyl)-lysine N-3-(methacrylamido)propylcarboxamide;
- and
- N-α-(3-boronato-5-nitro)benzoyl-N-ε-(4-dimethylamino-3,5-dinitro)benzoyllysine N-3-(methacrylamido)propyl-carboxamide.
Dependencies are inferred from the claim text.