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US 2002/0119573 A1Published application
Footprinting plasmid
OwnerKaren J. Shaw, Teresa Kenney +1inventors
InventorsKaren J. Shaw, Teresa Kenney, George H. Shimer JR.
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Abstract
The present invention provides plasmids for insertional mutagenesis in bacteria. Specifically, the invention provides plasmids comprising a transposition system including a transposon and a transposase, in which transposon insertion can be temporally regulated. The plasmids can be used to determine the relative importance of a particular gene in viability of the organism from which the gene is derived. The plasmids are particularly useful for large-scale screening of a multiplicity of genes.
Claims18
A plasmid comprising
- (i) a transposon carrying a selectable marker gene;
- (ii) a gene encoding a transposase that catalyzes insertion of said transposon into genomic DNA in vivo, wherein said gene is operably linked to a regulatable promoter;
- and
- (iii) an environmentally sensitive bacterial origin of replication.
1 more element
A plasmid comprising
- (i) a Tn10 transposon carrying a chloramphenicol resistance gene;
- (ii) a gene encoding Tn10 ATS transposase operably linked to a a PXYL promoter;
- (iv) a temperature sensitive bacterial origin of replication derived from pE194;
- and
- (iv) a bacterial origin of replication derived from pBR322.
2 more elements
Dependencies are inferred from the claim text.