Human oncogene induced secreted protein I
AssigneeHuman Genome Sciences, Inc.
InventorsHenrik S. Olsen, Steven M. Ruben
Life of this patent
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Abstract
The present invention relates to a novel protein, the Human Oncogene Induced Secreted Protein I (“HOIPS I”) protein. In particular, isolated nucleic acid molecules are provided encoding the human HOIPS I protein. HOIPS I polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. Also provided are diagnostic methods for detecting abnormal cell proliferation and differentiation disorders and therapeutic methods for treating the same.
Claims16
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding a polypeptide comprising amino acids from about −20 to about 142 in SEQ ID NO:2;
- (b) a nucleotide sequence encoding a polypeptide comprising amino acids from about −19 to about 142 in FIG. 1 SEQ ID NO:2;
- (c) a nucleotide sequence encoding a polypeptide comprising amino acids from about 1 to about 142 in SEQ ID NO:2;
- (d) a nucleotide sequence encoding a polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- (e) a nucleotide sequence encoding the mature HOIPS I polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- and
- (f) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d) or (e).
An isolated nucleic acid molecule comprising a polynucleotide having a sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) the nucleotide sequence of a fragment of the sequence shown in SEQ ID NO:
- 1, wherein said fragment comprises at least 50 contiguous nucleotides of SEQ ID NO:
- 1, provided that said nucleotide sequence is not SEQ ID NO:1, SEQ ID NO:12, SEQ ID NO:
- 13, SEQ ID NO:14, SEQ ID NO:15, or SEQ ID NO:16;
- and
- (b) a nucleotide sequence complementary to a nucleotide sequence in (a).
An isolated HOIPS I polypeptide having an amino acid sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) amino acids from about −20 to about 142 in SEQ ID NO:2;
- (b) amino acids from about −19 to about 142 in SEQ ID NO:2;
- (c) amino acids from about 1 to about 142 in SEQ ID NO:2;
- (d) the amino acid sequence of the HOIPS I polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- (e) the amino acid sequence of the mature HOIPS I polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- and
- (f) the amino acid sequence of an epitope-bearing portion of any one of the polypeptides of (a), (b), (c), (d), or (e).
An isolated polypeptide comprising an epitope-bearing portion of the HOIPS I protein, wherein said portion is selected from the group consisting of:
- a polypeptide comprising amino acid residues from about −4 to about 9 of SEQ ID NO:2, a polypeptide comprising amino acid residues from about 13 to about 19 of SEQ ID NO:2, a polypeptide comprising amino acid residues from about 23 to about 32 of SEQ ID NO:2, a polypeptide comprising amino acid residues from about 36 to about 47 of SEQ ID NO:2, a polypeptide comprising amino acid residues from about 54 to about 63 of SEQ ID NO:2, a polypeptide comprising amino acid residues from about 70 to about 74 of SEQ ID NO:2, a polypeptide comprising amino acid residues from about 90 to about 100 of SEQ ID NO:2, a polypeptide comprising amino acid residues from about 105 to about 119 of SEQ ID NO:2, and a polypeptide comprising amino acid residues from about 125 to about 132 of SEQ ID NO:2.
An isolated nucleic acid molecule comprising a polynucleotide encoding a HOIPS I polypeptide wherein, except for one to fifty conservative amino acid substitutions, said polypeptide has a sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding a polypeptide comprising amino acids from about −20 to about 142 in SEQ ID NO:2;
- (b) a nucleotide sequence encoding a polypeptide comprising amino acids from about −19 to about 142 in FIG. 1 SEQ ID NO:2;
- (c) a nucleotide sequence encoding a polyp eptide comprising amino acids from about 1 to about 142 in SEQ ID NO:2;
- (d) a nucleotide sequence encoding a polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- (e) a nucleotide sequence encoding the mature HOIPS I polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- and
- (f) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d) or (e).
An isolated HOIPS I polypeptide wherein, except for one to fifty conservative amino acid substitutions, said polypeptide has a sequence selected from the group consisting of:
- (a) amino acids from about −20 to about 142 in SEQ ID NO:2;
- (b) amino acids from about −19 to about 142 in SEQ ID NO:2;
- (c) amino acids from about 1 to about 142 in SEQ ID NO:2;
- (d) the amino acid sequence of the HOIPS I polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- (e) the amino acid sequence of the mature HOIPS I polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97825;
- and
- (f) the amino acid sequence of an epitope-bearing portion of any one of the polypeptides of (a), (b), (c), (d), or (e).
Dependencies are inferred from the claim text.