Genes encoding UMP kinase, methods for purifying UMP kinase and methods of characterizing UMP kinase
AssigneeInstitut Pasteur
InventorsOctavian Barzu, Ann-Marie Gilles, Hiroshi Sakamoto, Helene Munier-Lehmanni
Life of this patent
Estimating the term…
Abstract
The present invention relates to the identification of the pyrH/smbA gene which encodes the UMP kinase gene in B. subtilis, M. tuberculosis, and H. influenzae, which are activated, stablilized an inhibited by specific nucleotides, methods of purifying UMP kinase, methods of characterizing UMP kinase and methods of stablizing UMP kinase.
Claims18
An isolated polynucleotide comprising a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3, wherein said polynucleotide encodes a protein having UMP kinase activity.
A method of detecting the presence of a UMP kinase in a sample comprising
- adding GTP to said sample;
- and
- assaying for UMP kinase activity, wherein an increase in UMP kinase activity correlates to the presence of UMP kinase.
A method of detecting the presence of a UMP kinase in a sample comprising
- adding UTP to said sample;
- and
- assaying for UMP kinase activity, wherein a decrease in UMP kinase activity correlates to the presence of UMP kinase.
A method of detecting the presence of a UMP kinase in a sample comprising
- adding GTP to said sample;
- assaying for UMP kinase activity, wherein an increase in UMP kinase activity correlates to the presence of UMP kinase;
- adding UTP to said sample;
- and
- assaying for UMP kinase activity, wherein a decrease in UMP kinase activity correlates to the presence of UMP kinase.
A method of stabilizing a UMP kinase in a sample, comprising adding UTP to said sample.
A method of activating a UMP kinase in a sample, comprising adding GTP to said sample.
The recombinant bacteria strains deposited at the CNCM under the accession numbers I-2542, I12574 and I12579.
Dependencies are inferred from the claim text.