Flow cytometry reagent and system
AssigneeStreck Laboratories, Inc.
InventorsWayne L. Ryan, Paula B. Turpen
Life of this patent
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Abstract
An improved reagent system for preparation of cells for flow cytometry having a physiologically compatible salt solution composition including a lysing agent, an agent for minimizing white blood cell lysis, and optionally a preservative.
Claims38
A reagent composition for preparing leukocytes for cytometric analysis, comprising:
- a. a lipoprotein;
- and
- b. an agent for lysing erythrocytes for permitting cytometric analysis of said leukocytes.
A reagent composition for preparing leukocytes for analysis by flow cytometry, comprising:
- a. about 5 to about 100 mg/dl of lipoprotein cholesterol;
- b. about 10 to about 300 mg mg/dl of saponin;
- and
- c. about 1 to about 6 gm/dl of a preservative.
An aqueous reagent composition for preparing leukocytes for analysis by flow cytometry, comprising:
- a. about 0.01 to about 5 parts by weight high density lipoprotein;
- b. about 0.1 to about 2 parts by weight of saponin;
- c. up to about 5 parts by weight of diazolidinyl urea;
- and
- d. about 0.1 to about 2 parts by weight of a halide salt.
A method for preparing a blood sample for fluorescent analysis with a flow cytometer, comprising the steps of:
- a. contacting at least one leukocyte in said blood sample with an aqueous reagent that includes:
- i. a lipoprotein agent for resisting lysing of white blood cells;
- and
- ii. an effective amount of an agent for lysing erythrocytes;
- and
- iii. a physiologically compatible salt;
- b. labeling said at least one leukocyte with a fluorescent label associated with a known antibody;
- c. analyzing said at least one leukocyte with an analytical instrument.
A system for flow cytometry, comprising:
- a. a flow cytometer instrument;
- b. a reagent for preparing leukocytes for analysis by flow cytometry, said reagent including:
- i. an effective amount of a lipoprotein;
- and
- ii. an effective amount of a lytic agent.
Dependencies are inferred from the claim text.