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US 2002/0119491 A1Published application
High expression and production of high specific activity recombinant S-adenosyl homocysteinase (SAHH) and improved assays for S-adenosylmethionine (SAM)
AssigneeAntiCancer, Inc.
InventorsMingxu Xu, Qinghong Han
Life of this patent
Estimating the term…
Abstract
The invention provides novel methods relating to SAM detection and production as well as a novel SAHH enzymatic activity for use in such methods. Additional methods, compositions, and kits relating to the novel SAHH are also provided.
Claims21
A method to assess therapeutic levels of S-adenosylmethionine (SAM) in a biological fluid sample which method comprises
- providing said sample with glycine N-methyltransferase (GMT), a S-adenosyl homocysteine hydrolase (SAHH) or His•SAHH, and glycine;
- and
- measuring one or more reaction products in said sample wherein the level(s) of said one or more reaction products is directly proportional to the level of SAM in the sample.
A kit for assaying a sample containing SAM, the kit comprising SAHH or His•SAHH, GMT, glycine and instructions for use.
An assay comprising:
- a biological sample containing SAM;
- and
- GMT, glycine, and SAHH or His•SAHH,
- wherein SAHH or His•SAHH activity results in a product that is capable of being measured to determine the amount of SAM in the sample.
1 more element
An isolated nucleic acid molecule comprising SEQ ID NO:1.
Dependencies are inferred from the claim text.