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US 2002/0119485 A1Published application
Luminescence assays
AssigneePhotonic Research Systems Limited
InventorChristopher Morgan
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AssigneePhotonic Research Systems Limited
InventorChristopher Morgan
Estimating the term…
A method of performing an energy transfer luminescence assay to determine an analyte in which a donor species is irradiated to raise the donor species to an excited state, and the analyte causes a change in the excitation condition of at least one of the donor species and an acceptor species and said change is monitored to determine the analyte. The donor species is provided as, in or adsorbed to a solid phase and is an up-conversion medium capable of effecting, by energy pooling or otherwise, an energy transition to an excited state by absorption of electromagnetic radiation having an energy less than that of said transition. The acceptor species is bound or is capable of being bound directly or indirectly to the surface of said solid phase. In the method, the donor species is irradiated with said electromagnetic radiation and luminescence is detected in at least one spectral region characteristic of the emission of the donor species and/or the acceptor species provided that the excitation of the acceptor species to an excited state capable of luminescence in a defined spectral region of the acceptor species does not occur by absorption of a single quantum of the radiation used to excite the donor species.
A method of determining an analyte by means of luminescence assay in which
A method of detection of luminescence in which a luminescent species of interest is excited by a process of simultaneous or sequential absorption of at least two photons from at least one photon source, wherein the or each photon source is modulated at one or more frequencies, and luminescence measurements are made at a frequency or frequencies different from the or each modulation frequency, the or each frequency at which measurements are made being a function of the or each modulation frequency by virtue of the excitation process.
An apparatus for detecting luminescence in which a luminescent species is excited by a process of simultaneous or sequential absorption of at least two photons, comprising at least one photon source, means for modulating the or each photon source at a pre-determined frequency, means for detecting emissions at a frequency which is different from the or each modulation frequency but which is a function of the or each modulation frequency by virtue of the excitation process.
A composition comprising
A microarray analytical device comprising a plurality of spatially distinct microscopic regions each comprised of binding ligands wherein said ligands are provided on or in close proximity to the surface of an associated metal coating, said device further comprising donor species provided as, in or adsorbed to a solid phase and being an upconversion medium capable of effecting an energy transition to an excited state by absorption of electromagnetic radiation having an energy less than that of said excited state,, said metal coating being provided on the donor species.
Dependencies are inferred from the claim text.