Purification devices comprising immobilized capture probes and uses therefor
OwnerLawrence Weir, Christopher P. Adams +3inventors
InventorsLawrence Weir, Christopher P. Adams, T. Christian Boles, Rahul Dhanda, Stephen J. Kron
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Abstract
The present invention pertains to devices comprising at least one purification unit, used to purify and/or concentrate a target molecule contained within a test sample. Typically, the target molecule in a test sample will be a nucleic acid. These purified nucleic acids can be used in a variety of ways, including being subjected to nucleotide sequence analysis. Methods of using the devices and kits containing the devices are also provided.
Claims38
A device for purifying a target nucleic acid molecule from a test sample comprising at least one purification unit, the purification unit comprising:
- (a) a receptacle;
- and
- (b) an electrophoretic medium comprising at least one immobilized capture probe selected to hybridize to the target nucleic acid.
A method for purifying a target nucleic acid molecule from a test sample comprising the steps of:
- (a) introducing a test sample containing the target nucleic acid molecule into the receptacle of a unit of a purification device comprising:
- (1) a receptacle;
- and
- (2) an electrophoretic medium comprising at least one immobilized capture probe selected to hybridize to the target nucleic acid molecule;
- and
- (b) subjecting the electrophoretic medium to an electric field resulting in the migration of the test sample through the medium, under conditions suitable for the target molecule in the test sample to hybridize to the capture probe, thereby forming a target molecule/capture probe complex, and for the remaining components of the test sample to migrate through and elute from the medium.
A kit for preparing a target nucleic acid in a test sample for use in nucleic acid sequencing applications comprising a device for purifying a target nucleic acid from a test sample comprising at least one purification unit, the purification unit comprising:
- (a) a receptacle;
- and
- (b) an electrophoretic medium comprising at least one immobilized capture probe selected to hybridize to the preselected nucleic acid.
Dependencies are inferred from the claim text.