Nucleotide sequences coding for a mammalian coltage-gated potassium channel protein, the amino acid encoded by the nucleic acid and use thereof
OwnerVladimir I. Kashuba, Claes Wahlestedt +1inventors
InventorsVladimir I. Kashuba, Claes Wahlestedt, Eugene R. Zabarovsky
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Abstract
The present invention relates to the mammalian KCNA7 gene coding for a voltage-gated potassium channel protein. The invention further relates to methods for identifying agents capable of modulating voltage-gated potassium ion channel activity. Blockers of the KCNA7 ion channel would be expected i.a. to increase insulin release and thereby reduce hyperglycemia associated with non-insulin-dependent diabetes mellitus
Claims16
An isolated nucleic acid molecule selected from:
- (a) nucleic acid molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 or 11:
- (b) nucleic acid molecules comprising a nucleotide sequence capable of hybridizing, under stringent hybridization conditions, to a nucleotide sequence complementary the polypeptide coding region of a nucleic acid molecule as defined in (a) and which codes for a biologically active KCNA7 polypeptide or a functionally equivalent modified form thereof;
- and
- (c) nucleic acid molecules comprising a nucleic acid sequence which is degenerate as a result of the genetic code to a nucleotide sequence as defined in (a) or (b) and which codes for a KCNA7 polypeptide or a functionally equivalent modified form thereof.
An isolated human KCNA7 polypeptide having an amino acid sequence shown as SEQ ID NO:2 in the sequence listing.
An isolated murine kcna7 polypeptide having an amino acid sequence shown as SEQ ID NO:12 in the sequence listing.
A method for the identification of an agent modulating transcription of the human KCNA7 gene, comprising determining whether a candidate agent modulates expression of the human KCNA7 gene via a mechanism dependent upon a regulatory region shown as positions 105 to 114 or 201 to 211 in SEQ ID NO:13.
- A method for the identification of an agent modulating transcription of the human KCNA7 gene said method comprising the steps
- (i) contacting a candidate agent with a regulatory region shown as positions 105 to 114 or 201 to 211 in SEQ ID NO:13;
- and
- (ii) determining whether said candidate agent modulates expression of the human KCNA7 gene, such modulation being indicative for an agent modulating transcription of the human KCNA7 gene.
Dependencies are inferred from the claim text.