Method for increasing the processivity of a DNA- or RNA-dependent polymerase and compositions therefor

An improved method of cDNA synthesis, the improvement consisting in an addition of a RNA binding protein to the nucleic acid polymerization mixture comprising the reverse transcriptase, whereby said addition of general RNA binding protein enables an increase of the processivity of said reverse transcriptase, thereby enabling a significant increase in the production of full length cDNAs.

Use of a general RNA binding protein as an additive to improve the processivity of a nucleic acid-dependent polymerase, comprising an incubation of said polymerase in the presence of a processivity-improving amount of said RNA binding protein.

Use of a general RNA binding protein as an additive to improve the proportion of full length cDNA clones converting RNA to cDNA utilizing a reverse transcriptase, comprising an incubation of said reverse transcriptase in the presence of an effective amount of said general RNA binding protein.

A polymerization processivity-increasing composition, comprising a template nucleic acid, a polymerase and a general RNA binding protein, together with a suitable polymerization buffer.

A method to increase the processivity of RNA-dependent RNA polymerase comprising an addition of an effective amount of general RNA binding protein to a nucleic acid polymerization mixture comprising said RNA-dependent RNA polymerase, whereby said addition of general RNA binding protein enables an increase of the processivity of said polymerase.

A method to increase the processivity of a DNA-dependent DNA polymerase or DNA-dependent RNA polymerase comprising an addition of an effective amount of general DNA binding protein to a nucleic acid polymerization mixture comprising one of said polymerase, whereby said addition of a general DNA binding protein enables an increase of the processivity of one of said polymerase.

An improved method of cDNA synthesis, the improvement consisting in an addition of a DNA binding protein to the nucleic acid polymerization mixture comprising the DNA polymerase, whereby said addition of general DNA binding protein enables an increase of the processivity of said DNA polymerase during second strand systhesis, thereby enabling a significant increase in the production of full length cDNAs.

Use of a general DNA binding protein as an additive to improve the processivity of a nucleic acid-dependent polymerase, comprising an incubation of said polymerase in the presence of a processivity-improving amount of said general DNA binding protein.

Use of a general nucleic acid binding protein as an additive to improve the proportion of full length cDNA clones converting RNA to cDNA utilizing a reverse transcriptase, comprising an incubation of said reverse transcriptase in the presence of an effective amount of said general nucleic acid binding protein.

A method to increase the processivity of RNA-dependent DNA polymerase comprising an addition of an effective amount of general DNA binding protein to a nucleic acid polymerization mixture comprising a polymerase, whereby said addition of general DNA binding protein enables an increase of the processivity of said polymerase.