Back to results
US 2002/0119464 A1Published application
Compositions and methods enabling a totally internally controlled amplification reaction
AssigneeCepheid
InventorWilliam A. McMillan
Life of this patent
Estimating the term…
Abstract
The present invention provides compositions and methods for performing an amplification reaction of nucleic acids with internal controls that test the integrity of all aspects of the amplification reaction.
Claims20
A method of performing an amplification reaction, comprising:
- (a) combining in an aqueous solution:
- (i) a target probe, a first control probe and a second control probe;
- (ii) a first 5′ primer, a first 3′ primer and a target template, the target template comprising a hybridization site for the first 5′ primer, the first 3′ primer and the target probe;
- (iii) a first control template, the first control template comprising a hybridization site for the first 5′ primer, the first 3′ primer and the first control probe;
- and
- (iv) a second 5′ primer, a second 3′ primer and a second control template, the second control template comprising a hybridization site for the second 5′ primer, the second 3′ primer, the target probe and a second control probe;
- (b) performing an amplification reaction to create amplification products;
- and
- (c) quantifying binding of the target probe, first control probe and second control probe to the amplification products.
6 more elements
A kit comprising:
- (a) a target probe, a first control probe and a second control probe;
- (b) a first 5′ primer and a first 3′ primer;
- (c) a first control template, the first control template comprising a hybridization site for the first 5′ primer, the first 3′ primer and the first control probe;
- and
- (d) a second 5′ primer, a second 3′ primer and a second control template, the second control template comprising a hybridization site for the second 5′ primer, the second 3′ primer, the target probe and the second control probe.
2 more elements
A solution comprising:
- (a) a target probe, a first control probe and a second control probe;
- (b) a first 5′ primer and a first 3′ primer;
- (c) a first control template, the first control template comprising a hybridization site for the first 5′ primer, the first 3′ primer and the first control probe;
- and
- (d) a second 5′ primer, a second 3′ primer and a second control template, the second control template comprising a hybridization site for the second 5′ primer, the second 3′ primer, the target probe and the second control probe.
2 more elements
Dependencies are inferred from the claim text.