High fidelity polymerases and uses thereof

Abstract

The invention relates to a DNA and RNA polymerases which have increased fidelity (or reduced misincorporation rate). In particular, the invention relates to a method of making such polymerases by increasing or enhancing 3′-5′ exonuclease activity of a polymerase by, for example, substituting the 3′-5′ exonuclease domain of one polymerase with a 3′-5′ exonuclease domain with the desired activity from another polymerase. The invention also relates to DNA molecules containing the genes encoding the polymerases of the invention, to host cells containing such DNA molecules and to methods to make the polymerases using such host cells. The polymerases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.

Dependencies are inferred from the claim text.