Approaches to identify genetic traits
OwnerCharles L.M. Dunlop, James M. Weiselinventors
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Abstract
The present invention relates to the field of genetic screening. More specifically, the described embodiments concern methods to screen multiple samples, in a single assay, for the presence or absence of mutations or polymorphisms in a plurality of genes.
Claims67
A method of identifying the presence or absence of a plurality of genetic markers in a subject comprising:
- providing a DNA sample from said subject;
- providing a plurality of nucleic acid primer sets that hybridize to said DNA at regions that flank said plurality of genetic markers, wherein each primer set has a first and a second primer and, wherein said plurality of genetic markers exist on a plurality of genes;
- contacting said DNA and said plurality of nucleic acid primer sets in a single reaction vessel;
- generating, in said single reaction vessel, a plurality of extension products that comprise regions of DNA that include the location of said plurality of genetic markers;
- separating said plurality of extension products on the basis of melting behavior;
- and
- identifying the presence or absence of said plurality of genetic markers in said subject by analyzing the melting behavior of said plurality of extension products.
A method of identifying the presence or absence of a plurality of genetic markers in a plurality of subjects comprising:
- providing a DNA sample from said plurality of subjects;
- providing a plurality of nucleic acid primer sets that hybridize to said DNA at regions that flank said plurality of genetic markers, wherein each primer set has a first and a second primer and, wherein said plurality of genetic markers exist on a plurality of genes;
- contacting said DNA and said plurality of nucleic acid primer sets in a single reaction vessel;
- generating, in said single reaction vessel, a plurality of extension products that comprise regions of DNA that include the location of said plurality of genetic markers;
- separating said plurality of extension products on the basis of melting behavior;
- and
- identifying the presence or absence of said plurality of genetic markers in said plurality of subjects by analyzing the melting behavior of said plurality of extension products.
A method of identifying the presence or absence of a mutation or polymorphism in a subject comprising:
- providing a DNA sample from said subject;
- generating a population of extension products from said sample, wherein said extension products comprise a region of said DNA that corresponds to the location of said mutation or polymorphism;
- providing at least one control DNA, wherein said control DNA lacks said mutation or polymorphism;
- contacting said control DNA and said population of extension products in a single reaction vessel thereby forming a mixed DNA sample;
- heating said mixed DNA sample to a temperature sufficient to denature said control DNA and said DNA sample;
- cooling said mixed DNA sample to a temperature sufficient to anneal said control DNA and said DNA sample;
- separating said mixed DNA sample on the basis of melting behavior;
- and
- identifying the presence or absence of said mutation or polymorphism by analyzing the melting behavior of said mixed DNA sample.
An isolated or purified nucleic acid consisting of a sequence selected from the group consisting of SEQ. ID. Nos. 1-44.
A kit comprising an isolated or purified nucleic acid consisting of the sequence selected from the group consisting of SEQ. ID. Nos. 1-44.
A kit for performing amplification on a plurality of discrete genes of a subject, comprising:
- a mixture of at least 3 primer sets, each of said primer set adapted to amplify a DNA associated with a different genetic trait of said subject.
A reaction vessel comprising:
- a DNA sample obtained from a subject;
- and
- a plurality of nucleic acid primer sets that hybridize to said DNA sample at regions that flank a plurality of genetic markers, wherein said plurality of genetic markers exist on a plurality of genes.
A reaction vessel comprising:
- a plurality of DNA samples obtained from a plurality of subjects;
- and
- a plurality of nucleic acid primer sets that hybridize to said plurality of DNA samples at regions that flank a plurality of genetic markers, wherein said plurality of genetic markers exist on a plurality of genes.
A gel having lanes and adapted to separate different DNAs comprising:
- a plurality of extension products, in a single lane of said gel, wherein said plurality of extension products correspond to regions of DNA located on a plurality of genes and, wherein said regions of DNA comprise loci that indicate a genetic trait.
A gel having lanes and adapted to separate different DNAs comprising:
- a plurality of extension products, in a single lane of said gel, wherein said plurality of extension products correspond to regions of DNA located on a plurality of genes in a plurality of subjects and, wherein said regions of DNA comprise loci that indicate a genetic trait.
A denaturing high pressure liquid chromatography column adapted to separate different DNAs comprising:
- a plurality of extension products, wherein said plurality of extension products correspond to regions of DNA located on a plurality of genes and, wherein said regions of DNA comprise loci that indicate a genetic trait.
A gel having lanes and adapted to separate different DNAs comprising:
- a plurality of extension products, in a single lane of said gel, wherein said plurality of extension products correspond to regions of DNA located on a plurality of genes in a plurality of subjects and, wherein said regions of DNA comprise loci that indicate a genetic trait.
Dependencies are inferred from the claim text.