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OwnerAkihiro Suehara, Eiji Yamamoto +1inventors
InventorsAKIHIRO SUEHARA, EIJI YAMAMOTO, SHIGEO FUJII
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On the basis of differences in adsorbability to calcium phosphate gel formed by adding calcium ions to a Bordetella pertussis culture in the presence of excess phosphate ions, protective components of Bordetella pertussis are separated from the Bordetella pertussis culture. Traditionally, protective components of Bordetella pertussis have been separated using different purification methods for the respective components. According to the present invention, the use of the same means of purification for all subject components makes it possible to purify each component with high efficiency and high recovery rate, an aspect very advantageous for industrial production. It is also possible to efficiently produce an improved purified pertussis component vaccine comprising an effective combination of pertussis filamentous hemagglutinin (FHA), pertactin (PRN, 69K-OMP), pertussis fimbriae (FIM) and pertussis toxin (PT).
A method of separating at least one member of the group consisting of pertussis filamentous hemagglutinin (FHA), pertactin (PRN, 69K-OMP), pertussis fimbriae (FIM), and pertussis toxin (PT) by bringing a Bordetella pertussis culture into contact with calcium phosphate gel which is formed by adding calcium ions to the culture in the presence of phosphate ions.
A pertussis vaccine wherein the components PT:FHA:FIM are admixed in a ratio of 4-6:8-10:1.
A pertussis vaccine wherein the components PT:FHA:PRN:FIM are admixed in a ratio of 2-6:4-10:1-2:1.
Dependencies are inferred from the claim text.