KdtB

An isolated polynucleotide selected from the group consisting of:

• (i) an isolated polynucleotide comprising a polynucleotide sequence encoding a polypeptide that has at least 95% identity to the amino acid sequence of SEQ ID NO:
• 2, over the entire length of SEQ ID NO:
• 2;
• (ii) an isolated polynucleotide comprising a polynucleotide sequence that has at least 95% identity
• over its entire length to a polynucleotide sequence encoding the polypeptide of SEQ ID NO:
• 2;
• (iii) an isolated polynucleotide comprising a nucleotide sequence that has at least 95% identity
• to that of SEQ ID NO:
• 1 over the entire length of SEQ ID NO:
• 1;
• (iv) an isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO:
• 2;
• (v) an isolated polynucleotide that is the polynucleotide of SEQ ID NO:
• 1;
• (vi) an isolated polynucleotide of at least 30 nucleotides in length obtainable by screening an appropriate library under stringent hybridization conditions with a probe having the sequence of SEQ ID NO:
• 1 or a fragment thereof of of at least 30 nucleotides in length;
• (vii) an isolated polynucleotide encoding a mature polypeptide expressed by the kdtB gene comprised in the Streptococcus pneumoniae;
• and
• (viii) a polynucleotide sequence complementary to said isolated polynucleotide of (i), (ii), (iii), (iv), (v), (vi) or (vii).

A process for producing a polypeptide selected from the group consisting of:

• (i) an isolated polypeptide comprising an amino acid sequence selected from the group having at least 95% identity
• to the amino acid sequence of SEQ ID NO:
• 2 over the entire length of SEQ ID NO:
• 2;
• (ii) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
• 2;
• (iii) an isolated polypeptide that is the amino acid sequence of SEQ ID NO:
• 2, and
• (iv) a polypeptide that is encoded by a recombinant polynucleotide comprising the polynucleotide sequence of SEQ ID NO:
• 1,
• comprising the step of culturing a host cell under conditions sufficient for the production of the polypeptide.

A process for producing a host cell comprising an expression system or a membrane thereof expressing a polypeptide selected from the group consisting of:

• (i) an isolated polypeptide comprising an amino acid sequence selected from the group having at least 95% identity
• to the amino acid sequence of SEQ ID NO:
• 2 over the entire length of SEQ ID NO:
• 2;
• (ii) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
• 2;
• (iii) an isolated polypeptide that is the amino acid sequence of SEQ ID NO:
• 2, and
• (iv) a polypeptide that is encoded by a recombinant polynucleotide comprising the polynucleotide sequence of SEQ ID NO:
• 1,
• said process comprising the step of transforming or transfecting a cell with an expression system comprising a polynucleotide capable of producing said polypeptide of (i), (ii), (iii) or (iv) when said expression system is present in a compatible host cell such the host cell, under appropriate culture conditions, produces said polypeptide of (i), (ii), (iii) or (iv).

A host cell or a membrane expressing a polypeptide selected from the group consisting of:

• (i) an isolated polypeptide comprising an amino acid sequence selected from the group having at least 95% identity to the amino acid sequence of SEQ ID NO:
• 2 over the entire length of SEQ ID NO:
• 2;
• (ii) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
• 2;
• (iii) an isolated polypeptide that is the amino acid sequence of SEQ ID NO:
• 2, and
• (iv) a polypeptide that is encoded by a recombinant polynucleotide comprising the polynucleotide sequence of SEQ ID NO:
• 1.