Nucleotide sequences which code for the truB gene
OwnerMike Farwick, Klaus Huthmacher +2inventors
InventorsMike Farwick, Klaus Huthmacher, Walter Pfefferle, Brigitte Bathe
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Abstract
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the truB gene, and a host-vector system having a coryneform host bacterium in which the truB gene is present in attenuated form and a vector which carries at least the truB gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
Claims29
An isolated polynucleotide from coryneform bacteria, comprising a polynucleotide sequence which codes for the truB gene, selected from the group consisting of:
- a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2,
- b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2,
- c) polynucleotide which is complementary to the polynucleotides of a) or b), and
- d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c).
A coryneform bacteria in which the truB gene is enhanced.
An Escherichia coli strain DH5alphamcr/pEC-XK99EtruBex deposited as DSM 14460.
A method for the fermentative preparation of L-amino acids in coryneform bacteria, comprising:
- a) fermenting, in a medium, the coryneform bacteria which produce the desired L-amino acid and in which at least the endogenous truB gene or nucleotide sequences which code for it are enhanced.
Dependencies are inferred from the claim text.