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US 2002/0102575 A1Published application
In vitro amplification of nucleic acid molecules via circular replicons
AssigneeReplicon, Inc.
InventorJeffrey I. Auerbach
Life of this patent
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Abstract
Methods and compositions suitable for accomplishing the in vitro amplification of nucleic acid molecules via enzymatic means are provided. The preferred means employ circular rather than linear replicons. Means for producing such circular replicons from linear reactants are also provided.
Claims20
A composition for amplifying in vitro a target polynucleotide region of an initial linear nucleic acid molecule, wherein said composition comprises:
- (A) a single-stranded first polynucleotide, wherein said polynucleotide (i) contains a polynucleotide region that is complementary in sequence to said target polynucleotide region, and (ii) is a circular polynucleotide or is circularizable when hybridized to said target polynucleotide region in vitro;
- and
- (B) a second polynucleotide comprising said target polynucleotide region.
A kit for amplifying in vitro a target polynucleotide region of an initial linear nucleic acid molecule, wherein said kit comprises:
- (A) a first container, said first container containing a single-stranded first polynucleotide, wherein said polynucleotide (i) contains a polynucleotide region that is complementary in sequence to said target polynucleotide region, and (ii) is a circular polynucleotide or is circularizable when hybridized to said target polynucleotide region;
- and
- (B) a second container, said second container containing a second polynucleotide comprising said target polynucleotide region.
Dependencies are inferred from the claim text.