Methods for the preparation and use of internal standards for nucleic acid amplification assays
AssigneeBaxter Aktiengesellschaft
InventorsKlaus Zimmermann, Peter Turecek, Hans-Peter Schwarz, Manfred Rieger
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Abstract
Internal nucleic acid standards for nucleic acid amplification assays are provided. Specifically, internal nucleic acid standards are provided that are prepared using non-recombinant DNA technology. These internal nucleic acid standards are generally chemically synthesized and have a minimum size of approximately 90 nucleic acid bases. Also provided are internal nucleic acid standards prepared using non-recombinant DNA techniques that are single stranded nucleic acids.
Claims16
An internal control for a nucleic acid amplification assay comprising:
- a synthetic nucleic acid wherein said internal control is made using non-recombinant DNA techniques.
A method for the detection of nucleic acids in a sample, comprising:
- providing a sample;
- adding at least one internal nucleic acid standard to the sample;
- amplifying nucleic acids present in said sample;
- and
- detecting said amplified nucleic acids;
- wherein said at least one internal standard is an oligonucleotide synthesized using non-recombinant DNA techniques.
Dependencies are inferred from the claim text.