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AssigneeCampina Melkunie B.V.
InventorsLeon Franciscus Mallee, Ram Nimmagudda, Johannes Wilhelmus L. Boumans
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Described is a method for the preparation of a mixture of peptides having a cysteine content between 7-20 w/w % from a protein source, comprising cysteine containing proteins, comprising the steps of: a) cleaving the proteins of the protein source into peptides; b) digesting the peptides obtained in step a) by an exopeptidase, the action of which is at least attenuated at the position of a cysteine in the peptide, therewith forming digested peptides having a terminal cysteine; c) purifying the digested peptides, and the use of the preparation as active component in a medicament, especially for the treatment of conditions mediated by oxidative damage and for the elevation of cellular glutathion levels in the human or animal body.
Method for the preparation of a mixture of peptides having a cysteine content between 7-20 w/w % from a protein source, comprising cysteine containing proteins, comprising the steps of:
Method for the preparation of a mixture of peptides having a total cysteine and glycine content of 7-20 w/w % from a protein source, comprising cysteine and glycine containing proteins, comprising the steps of:
Method according to any of the preceding claims, wherein the at least one exopeptidase comprises carboxypeptidase Y.
Method according to any of the preceding claims, wherein the protein source comprises at least two different cysteine-containing proteins.
Method according to any of the preceding claims, wherein the protein source comprises at least two different proteins, at least one of which contains cysteine residues and at least one of which contains glycine residues.
Method according to any of the preceding claims, wherein the protein source consists of edible proteins.
Method according to any of the preceding claims, wherein the protein source comprises whey protein isolate and/or whey protein concentrate.
Method according to any of the preceding claims, wherein the protein source comprises one or more of the group, consisting of albumin, especially αlactalbumin, bovine serum albumin, wheat gluten, maize protein isolate, egg proteins, especially ovalbumin, cystatin.
Method according to any of the preceding claims, wherein step a) and step b) are done at conditions, wherein sulfur bridges between cysteine residues as present in the proteins of the protein source are kept intact.
Method according to any of the preceding claims, wherein step a) comprises cleavage of the proteins by an enzyme with endo-peptidase function.
Preparation comprising cysteine-rich peptides, comprising 7-20 w/w % cysteine.
Preparation comprising cysteine- and glycine-rich peptides, comprising 7-20 w/w % cysteine and glycine.
Dependencies are inferred from the claim text.