Photoactivatable nucleic acid derivatives
AssigneeSurModics, Inc.
InventorsPATRICK E. GUIRE, MELVIN J. SWANSON, GARY W. OPPERMAN
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Abstract
A photoactivatable nucleic acid derivative composition in which one or more photoreactive group(s) are bound to a natural or synthetic nucleic acid. The photoreactive groups can be bound to the nucleic acid before, during or after its formation, and can thereafter be activated in order to attach the nucleic acid to another molecule, e.g., to the surface of a solid support. Also described is a method of preparing such a composition, and a method of using such a composition to attach the nucleic acid to a another molecule, such as that provided by the surface of a substrate used to prepare a nucleic acid chip by photolithographic techniques.
Claims24
A composition comprising a photoactivatable nucleic acid derivative comprising a nucleic acid having one or more photoreactive groups bound thereto, wherein the photoreactive groups each generate an active species selected from the group consisting of nitrenes, carbenes, and excited states of ketones upon absorption of electromagnetic energy.
A method of preparing a composition comprising a photoactivatable nucleic acid derivative, the method comprising the steps of derivatizing a nucleic acid with one or more photoreactive groups, wherein the photoreactive groups each generate an active species selected from the group consisting of nitrenes, carbenes, and excited states of ketones upon absorption of electromagnetic energy.
A method of preparing a photoactivatable nucleic acid derivative, the method comprising the steps of covalently attaching one or more photoreactive groups to a synthetic oligonucleotide in the course of oligonucleotide synthesis, wherein the photoreactive groups each generate an active species selected from the group consisting of nitrenes, carbenes, and excited states of ketones upon absorption of electromagnetic energy
A probe array comprising a plurality of nucleic acids covalently attached thereto, wherein the nucleic acids are covalently attached via the residues of activated photoreactive groups.
Dependencies are inferred from the claim text.