Analysis method and cuvette therefor
AssigneeMigrata U.K. Ltd.
InventorsJan Lilja, Sven-Erik Nilsson, Johnny Svensson, Annika Eriksson
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Abstract
The present invention concerns a method for hemoglobin determination which comprises the steps of introducing a sample of undiluted whole blood by capillary action into a disposable microcuvette having at least one cavity for receiving the sample. The cavity includes a dry essentially non-hygroscopic hemolysing agent, which is dissolved by the blood, hemolyses the red blood cells and releases the haemoglobin contained in the blood cells. A first absorption measurement at a wavelength range 490-520 nm is then performed directly on the sample in the microcuvette, and a second absorption measurement is performed to compensate for background interference.
Claims14
A method for quantitative haemoglobin determination in undiluted whole blood comprising the steps of:
- introducing a sample of undiluted whole blood by capillary action into a disposable microcuvette having at least one cavity for receiving the sample, the cavity including an essentially non-hygroscopic hemolysing agent in a dry form, whereby the hemolysing agent is dissolved, hemolyses the red blood cells and releases the haemoglobin contained in the blood cells;
- performing a first absorption measurement at a wavelength range 490-520 nm directly on the hemolysed sample in the cuvette, and
- further conducting a second absorption measurement to compensate for background interference.
The method according to any one of the preceding claims wherein the hemolysing agent is selected in such a way that this agent in the dry form present in the ready-to-use microcuvette, permits a rapid introduction of whole blood into the cuvette.
The method according to claim any one of the preceding claims wherein the hemolysing agent is selected from the group consisting of sodium deoxycholate, potassium deoxycholate, calcium deoxycholate, morfolin deoxycholate, cyclohexylammonium deoxycholate, and ammonium deoxycholate or mixtures thereof.
The method according to claim any one of the preceding claims wherein the hemolysing agent essentially consists of a mixture of sodium deoxycholate and ammonium deoxycholate.
The method according to any of the preceding claims wherein the first absorption measurement is performed in the range 490-520, preferably 500-510 nm.
The method according to any of the preceding claims wherein the second absorption measurement is performed in the range 850-910 nm, preferably in the range 860-900 nm.
Disposable microcuvette for spectrophotometeric determination of haemoglobin in undiluted whole blood, characterised in that the cavity includes a dried, non hygroscopic hemolysing agent or a combination thereof provided that the cavity is essentially free from azide and nitrite.
Dependencies are inferred from the claim text.