Cell growth method and device with multiple applications
OwnerLuc Douay, Jean-Luc Magalhaes +1inventors
InventorsLuc Douay, Jean-Luc Magalhaes, Florence Hummel
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Abstract
A cell growth method and device with multiple applications, in particular to the maintenance, proliferation, amplification or differentiation of cells in a closed enclosure (10), including individual flow rate or quantity adjustment means (30, 36) for determining compositions and flow rates of mixtures of different nutrient media or constituents and compositions and flow rates of different mixtures of growth factors, corresponding to an envisaged application, and then to modify the compositions and/or the flow rates of the mixtures of nutrient media or constituents and of the mixtures of growth factors delivered to the enclosure, which modifications can be effected independently of each other.
Claims9
A method of growing cells, which method has multiple applications, in particular to the maintenance, proliferation, amplification or differentiation of cells in a closed enclosure (10), and consists of introducing cells into said enclosure, delivering to said enclosure ventilation gases, nutrient media and growth factors, and harvesting the cultivated cells, characterized in that it further consists of:
- providing multiple sources (28) of different nutrient media or constituents and multiple sources (34) of different growth factors,
- determining compositions and flow rates of mixtures of the different nutrient media or constituents and compositions and flow rates of mixtures of the different growth factors corresponding to an envisaged application, and then
- during the progress of said application, modifying at regular or irregular intervals the compositions and/or the flow rates of the mixtures of nutrient media or constituents and the mixtures of growth factors delivered to the enclosure (10), which modifications can be effected independently of each other.
A device for growing cells, which device has multiple applications, in particular to the maintenance, proliferation, amplification or differentiation of cells, and includes a closed enclosure (10), microporous membranes (14) delimiting a growth chamber (12) in said enclosure, means (44) for delivering cells to said chamber, means for delivering ventilation gases, nutrient media and growth factors to the enclosure, and means (44) for harvesting cells, characterized in that it further includes containers (28) of different nutrient media or constituents connected by flow rate adjustment means (30) to a delivery manifold (32) of said enclosure, containers (34) of different growth factors connected by flow rate adjustment means (36) to another delivery manifold or the same delivery manifold (38, 32) of the enclosure, and means (52) for controlling the aforementioned adjustment means (30, 36) to determine, as a function of an envisaged application, the composition and/or the flow rate of a mixture of nutrient media and the composition and/or the flow rate of a mixture of growth factors delivered to the enclosure via said delivery manifold or manifolds and to modify said compositions and/or said flow rates as said application proceeds.
Dependencies are inferred from the claim text.