Novel epidermis/dermis equivalents and aged skin equivalents shaped therefrom

Epidermis equivalent comprising at least keratinocytes, said epidermis equivalent being obtained by seeding of at least keratinocytes onto a dermis equivalent comprising at least glycated collagen and fibroblasts.

Epidermis equivalent comprising at least keratinocytes, said epidermis equivalent having modified expression of β1 integrin.

Epidermis equivalent comprising at least keratinocytes, said epidermis equivalent having modified expression of β1 integrin, said epidermis equivalent being obtained by seeding of at least keratinocytes onto a dermis equivalent comprising at least glycated collagen and fibroblasts.

Epidermis equivalent comprising at least keratinocytes, said epidermis equivalent having β1 integrin expression in the cells of at least the first three suprabasal layers, said epidermis equivalent being obtained by seeding of at least keratinocytes onto a dermis equivalent comprising at least glycated collagen and fibroblasts.

Aged skin equivalent, comprising at least one epidermis equivalent and one aged dermis equivalent.

A method for preparing an aged skin equivalent comprising an epidermis equivalent and an aged dermis equivalent which comprises a lattice comprising at least glycated collagen and fibroblasts, said method comprising, in a first step, preparing a lattice comprising at least glycated collagen and fibroblasts and, in a second step, reconstructing an epidermis equivalent comprising at least keratinocytes onto the lattice obtained in the first step.