Method and apparatus for hybridization
AssigneeAffymetrix, Inc.
InventorsDonald M. Besemer, Virginia W. Goss, James L. Winkler
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Abstract
A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
Claims27
A method of making probe chips comprising the steps of:
- forming a plurality of probe arrays on a substrate;
- separating said substrate into a plurality of chips, each of said chips comprising at least one probe array thereon;
- and
- mating at least one of said chips to a package, said package comprising a reaction chamber, said reaction chamber comprising inlets for flowing fluid therein, said at least one probe array in fluid communication with said reaction chamber.
An apparatus for packaging a substrate, said apparatus comprising:
- a substrate having a first surface and a second surface, said first surface comprising a probe array;
- a body having a mounting surface with a fluid cavity, said second surface attached to said cavity;
- and
- a cover attached to said mounting surface for sealing said cavity.
An apparatus for packaging a substrate, said apparatus comprising:
- a substrate having a first surface and a second surface, said first surface comprising a probe array and said second surface being an outer periphery of said first surface;
- a body having a mounting surface, an upper surface, and a cavity bounded by said mounting surface and said upper surface, said second surface being attached to said cavity and said first surface being within said cavity;
- and
- a cover attached to said mounting surface for defining an upper boundary to said cavity;
- wherein said cavity comprises a diffuser and a concentrator, said diffuser and said concentrator permitting laminar fluid flow through said cavity.
Dependencies are inferred from the claim text.