DNA molecules encoding fibrinolytically active polypeptide
AssigneeAmgen Inc.
InventorsThomas C. Boone, Huimin Li, Michael B. Mann
Life of this patent
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Abstract
A fibrinolytically active metalloproteinase polypeptide (called “novel acting thrombolytic”) which is useful for blood clot lysis in vivo and methods and materials for its production by recombinant expression are described.
Claims19
A fibrinolytically active polypeptide having the amino acid sequence of SEQ ID NO:
- 1.
A nucleic acid molecule encoding the polypeptide of SEQ ID NO:
- 1.
A method for the production of NAT, comprising culturing host cells containing a DNA molecule encoding said metalloproteinase operatively linked to regulatory elements for stimulating the expression thereof under conditions such that the DNA molecule is expressed, and isolating the expressed metalloproteinase from the host cell culture.
A method for the production of a metalloproteinase fibrinolytic agent selected from the group consisting of fibrolase and NAT, comprising culturing Pichia host cells containing a DNA molecule encoding said metalloproteinase operatively linked to regulatory elements for stimulating the expression thereof under conditions such that the DNA molecule is expressed, and isolating the expressed metalloproteinase from the host cell culture.
A method for the treatment of thrombosis in a mammal comprising administering to the mammal a thrombolytically effective amount of NAT.
Dependencies are inferred from the claim text.