Method of determining the activity of 1-deoxy-D-xylulose-5-phosphate reductoisomerase and 1-deoxy-D-xylulose-5-phosphate synthase
OwnerWilliam Frank Martin, Ruediger Hain +3inventors
InventorsWilliam Frank Martin, Ruediger Hain, Klaus-Guenther Tietjen, Marco Busch, Andreas S. Kloti
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Abstract
The invention relates to DNA which encodes Arabidopsis 1-eoxy-xylulose-5-phosphate reductoisomerase and to a method of identifying modulators of the 1-deoxy-D-xylulose-5-phosphate reductoisomerase and 1-deoxy-D-xylulose-5-phosphate synthase activity.
Claims21
DNA which encodes Arabidopsis 1-deoxy-D-xylulose-5-phosphate reductoisomerase or functional fragments thereof.
DNA, characterized in that its homology to the sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 5 is at least 80%.
Use of 1-deoxy-D-xylulose-5-phosphate reductoisomerase and/or 1-deoxy-D-xylulose-5-phosphate synthase modulators as herbicides, antibiotic agents or anti-malarial agents.
Method of determining the activity of 1-deoxy-D-xylulose-5-phosphate reducloisomerase and/or 1-deoxy-D-xylulose-5-phosphate synthase, characterized in that
- (a) pyruvate and glyceraldehyde-3-phosphate are converted into 1-deoxy-D-xylulose-5-phosphate with the aid of 1-deoxy-D-xylulose-5-phosphate synthase, and
- (b) the resulting 1-deoxy-D-xylulose-5-phosphate is converted into 2-C-methyl-D-erythrol4-phosphate with the aid of 1-deoxy-D-xylulose-5-phosphate reductoisomerase.
Polypeptide represented by SEQ ID NO. 2 or SEQ ID NO. 6.
Dependencies are inferred from the claim text.