Fungal antigens and process for producing the same
AssigneeTakara Shuzo Co., Ltd.
InventorsKazutoh Takesako, Shigetoshi Mizutani, Masahiro Endo, Ikunoshin Kato
Life of this patent
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Abstract
There can be provided a fungal antigen which is an insoluble fraction obtainable from fungal cells of which cell wall has been substantially removed or at least partially removed; a process for producing the same; a nucleic acid encoding the fungal antigen; a biologic product containing the fungal antigen; a method of stimulating immunological responses by using the biologic product; a method of suppressing allergic reaction to fungi in a vertebrate; and a method for diagnosing a disease caused by fungi in a vertebrate.
Claims20
A nucleic acid encoding a fungal antigen selected from the group consisting of:
- (a) a fungal antigen comprising an antigenic protein having a vaccine activity or an allergen activity originated from Candida albicans, wherein said antigenic protein comprises the partial amino acid sequence as shown by SEQ ID NO:1 in Sequence Listing and has a molecular weight of about 65,000 as determined by SDS-PAGE under reduced conditions,
- (b) a fungal antigen comprising a peptide comprising an entire sequence of the amino acid sequence as shown by SEQ ID NO:5 in Sequence Listing, or a partial sequence thereof, the peptide having a vaccine activity or an allergen activity, and
- (c) a fungal antigen comprising a peptide resulting from at least one of deletion, addition, insertion or substitution of one or more amino acid residues in the amino acid sequence as shown by SEQ ID NO:5 in Sequence Listing, or a partial sequence thereof, the peptide having a vaccine activity or an allergen activity.
A nucleic acid encoding a fungal antigen selected from the group consisting of:
- (d) a fungal antigen comprising an antigenic protein having a vaccine activity or an allergen activity originated from Candida albicans, wherein said antigenic protein comprises the partial amino acid sequence as shown by SEQ ID NO:2 in Sequence Listing and has a molecular weight of about 25,000 as determined by SDS-PAGE under reduced conditions,
- (e) a fungal antigen comprising a peptide comprising an entire sequence of the amino acid sequence as shown by SEQ ID NO:6 in Sequence Listing, or a partial sequence thereof, the peptide having a vaccine activity or an allergen activity, and
- (f) a fungal antigen comprising a peptide resulting from at least one of deletion, addition, insertion or substitution of one or more amino acid residues in the amino acid sequence as shown by SEQ ID NO:6 in Sequence Listing, or a partial sequence thereof, the peptide having a vaccine activity or an allergen activity.
A process for producing a fungal antigen which is an insoluble fraction obtainable from fungal cells of which cell wall has been substantially removed or at least partially removed, characterized in that said process comprises the steps of:
- (1) obtaining living fungal cells;
- (2) obtaining fungal cells of which cell wall has been substantially removed or at least partially removed;
- (3) bursting the fungal cells of which cell wall has been substantially removed or at least partially removed;
- and
- (4) obtaining an insoluble fraction.
A process for producing a fungal antigen which is a solubilized fraction extracted and separated from an insoluble fraction obtainable from fungal cells of which cell wall has been substantially removed or at least partially removed, characterized in that said process comprises the steps of:
- (1) obtaining living fungal cells;
- (2) obtaining fungal cells of which cell wall has been substantially removed or at least partially removed;
- (3) bursting the fungal cells of which cell wall has been substantially removed or at least partially removed;
- (4) obtaining an insoluble fraction;
- and
- (5) extracting and separating a solubilized fraction from the insoluble fraction.
Dependencies are inferred from the claim text.