Metal chelate containing compositions for use in chemiluminescent assays
AssigneeDade Behring Marburg GmbH
InventorsSharat Singh, Edwin F. Ullman
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Abstract
Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.
Claims44
A composition comprising:
- (a) a metal chelate comprising a metal selected from the group consisting of europium, terbium, dysprosium, samarium, osmium and ruthenium in at least a hexacoordinated state and
- (b) a compound having a structural portion that is a double bond substituted with two aryl groups, wherein one of the aryl groups is electron donating with respect to the other, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen.
A compound of the formula:
- 42
- wherein X′ is S or NR′ wherein R′ is alkyl or aryl and D and D′ are independently selected form the group consisting of alkyl and alkyl radical.
A composition comprising a latex having incorporated therein a compound of the formula:
- 43
- wherein X″ is O, S or NR″ wherein R″ is alkyl or aryl, n is 1 to 4, and Ar and Ar′ are independently aryl wherein one of Ar or Ar′ is electron donating with respect to the other and Y is hydrogen or an organic radical consisting of atoms selected from the group consisting of C, O, N, S, and P and m is 0 to 2.
A composition comprising a latex having incorporated therein a compound of the formula:
- 44
- wherein X′ is S or NR′ wherein R′ is alkyl or aryl and D and D′ are independently selected from the group consisting of alkyl and alkyl radical.
A method for determining an analyte which comprises:
- (a) providing in combination (1) a medium suspected of containing an analyte, (2) a photosensitizer capable in its excited state of activating oxygen to a singlet state, said photosensitizer associated with a specific binding pair (sbp) member, and (3) a suspendible latex particulate material comprising a chemiluminescent compound, said particulate material having bound thereto an sbp member, said chemiluminescent compound having the formula:
- 45
- wherein X″ is O, S or NR″ wherein R″ is alkyl or aryl, n is 1 to 4, and Ar and Ar′ are independently aryl wherein one of Ar or Ar′ is electron donating with respect to the other and Y is hydrogen or an organic radical consisting of atoms selected from the group consisting of C, O, N, S, and P and m is 0 to 2,
- (b) treating said combination with light to excite said photosensitizer, and
- (c) examining said combination for the amount of luminescence emitted therefrom, the amount of said luminescence being related to the amount of analyte in said medium.
A method for determining an analyte which comprises:
- (a) providing in combination (1) a medium suspected of containing an analyte, (2) a photosensitizer capable in its excited state of activating oxygen to a singlet state, said photosensitizer associated with a specific binding pair (sbp) member, and (3) a suspendible latex particulate material comprising a chemiluminescent compound, said particulate material having bound thereto an sbp member, said chemiluminescent compound having the formula:
- 46
- wherein X′ is S or NR′ wherein R′ is alkyl or aryl and D and D′ are independently selected from the group consisting of alkyl and alkyl radical.
- (b) treating said combination with light to excite said photosensitizer, and
- (c) examining said combination for the amount of luminescence emitted therefrom, the amount of said luminescence being related to the amount of analyte in said medium.
A kit comprising in packaged combination:
- (1) a composition comprising a suspendible latex particle comprising a chemiluminescent compound of the formula:
- 47
- wherein X″ is O, S or NR″ wherein R″ is alkyl or aryl, n is 1 to 4, and Ar and Ar′ are independently aryl wherein one of Ar or Ar′ is electron donating with respect to the other and Y is hydrogen or an organic radical consisting of atoms selected from the group consisting of C, O, N, S, and P and m is 0 to 2, said particle having bound thereto a specific binding pair (sbp) member, and (2) a photosensitizer capable in its excited state of activating oxygen to its singlet state.
Dependencies are inferred from the claim text.