Detection of microsatellite instability and its use in diagnosis of tumors
AssigneePromega Corporation
InventorsJeffery W. Bacher, Laura Flanagan, Nadine Nassif
Life of this patent
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Abstract
Methods and kits are disclosed for use in the analysis of microsatellite instability in genomic DNA. Methods and kits are also disclosed which can be used to detect microsatellite instability DNA present in biological materials, such as tumors. The methods and kits of the present invention can be used to detect or diagnose diseases associated with microsatellite instability, such as certain types of cancer.
Claims71
A method of analyzing micro-satellite loci, comprising steps of:
- a) providing primers for co-amplifying a set of at least three microsatellite loci of genomic DNA, comprising at least one mononucleotide repeat locus selected from the group consisting of BAT-25, BAT-26, BAT-40, MONO-11, and MONO-15, and at least two tetranucleotide repeat loci selected from the group consisting of FGA, D1S518, D1S547, D1S1677, D2S1790, D3S2432, D5S818, D5S2849, D6S1053, D7S3046, D7S1808, D7S3070, D8S1179, D9S2169, D10S1426, D10S2470, D12S391, D17S1294, D17S1299, and D18S51;
- b) co-amplifying the set of at least three microsatellite loci from at least one sample of genomic DNA in a multiplex amplification reaction, using the primers, thereby producing amplified DNA fragments;
- and
- c) determining the size of the amplified DNA fragments.
A method of detecting microsatellite instability in genomic DNA, comprising the steps of:
- a) providing primers for co-amplifying a set of at least three microsatellite loci of genomic DNA, comprising at least one mononucleotide repeat locus selected from the group consisting of BAT-25, BAT-26, BAT-40, MONO-11, and MONO-15, and at least two tetranucleotide repeat loci selected from the group consisting of FGA, D1S518, D1S547, D1S1677, D2S1790, D3S2432, D5S818, D5S2849, D6S1053, D7S3046, D7S1808, D7S3070, D8S1179, D9S2169, D10S1426, D10S2470, D12S391, D17S1294, D17S1299, and D18S51;
- b) co-amplifying the set of at least three microsatellite loci from a first sample of genomic DNA originating from normal non-cancerous biological material from an individual and a from a second sample of genomic DNA originating from a second biological material from the individual, in separate multiplex amplification reactions, using at least one oligonucleotide primer for each of the microsatellite loci, thereby producing first amplified DNA fragments from the first sample and second amplified DNA fragments from the second sample;
- and
- c) comparing the size of first amplified DNA fragments to the size of the second amplified DNA fragments to detect instability in any of the at least three microsatellite loci of the second genomic DNA.
A kit for analyzing microsatellite loci of genomic DNA, comprising:
- a single container with oligonucleotide primers for co-amplifying a set of at least three microsatellite loci of genomic DNA, the set comprising one mononucleotide repeat locus selected from the group consisting of BAT-25, BAT-26, BAT-40, MONO-11, and MONO-15, and at least two tetranucleotide repeat loci selected from the group consisting of FGA, D1S518, D1S547, D1S1677, D2S1790, D3S2432, D5S818, D5S2849, D6S1053, D7S3046, D7S1808, D7S3070, D8S1179, D9S2169, D10S1426, D10S2470, D12S391, D17S1294, D17S1299, and D18S51.
A method of analyzing micro-satellite loci, comprising steps of:
- a) providing primers for co-amplifying a set of at least nine microsatellite loci of human genomic DNA, comprising BAT-25, BAT-26, MONO-15, BAT-40, D3S2432, D7S3046, D7S3070, D7S1808, and D10S1426;
- b) co-amplifying the set of at least three microsatellite loci from at least one sample of genomic DNA in a multiplex amplification reaction, using the primers, thereby producing amplified DNA fragments;
- and
- c) determining the size of the amplified DNA fragments.
A kit for analyzing microsatellite loci of genomic DNA, comprising:
- a single container with oligonucleotide primers for co-amplifying a set of at least nine microsatellite loci of human genomic DNA, comprising BAT-25, BAT-26, MONO-15, BAT-40, D3S2432, D7S3046, D7S3070, D7S1808, and D10S1426.
A method of analyzing micro-satellite loci, comprising steps of:
- a) providing primers for co-amplifying a set of at least three microsatellite loci of genomic DNA, comprising at least one mono-nucleotide repeat locus and at least two tetra-nucleotide repeat loci;
- b) co-amplifying t he set of a t least three microsatellite loci from at least one sample of genomic DNA in a multiplex amplification reaction, using the primers, thereby producing amplified DNA fragments;
- and
- c) determining the size of the amplified DNA fragments.
A kit for analyzing microsatellite loci of human genomic DNA, comprising:
- a single container with oligonucleotide primers for co-amplifying a set of at least three microsatellite loci of human genomic DNA, the set comprising one mono-nucleotide repeat locus and two tetra-nucleotide repeat loci.
Dependencies are inferred from the claim text.