PCR reaction mixture for fluorescence-based gene expression and gene mutation analyses
AssigneeFraunhofer-Gesellschaft zur Forderung der angewandten Forshung e.V.
InventorsThomas Thum, Jurgen Borlak
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Abstract
An increase in the selectivity, sensitivity and the suppression of primer dimer formations, fluorescence-based gene expression analyses and gene mutation analyses is accomplished by adding bovine serum albumin to the conventional PCR reaction components. Magnesium chloride concentration is adjusted accurately depending on the Taq polymerase used.
Claims26
A PCR reaction mixture for fluorescence-based gene expression analyses and gene mutation analyses, wherein the reaction mixture, aside from the usual reaction components, additionally contains bovine serum albumin and has an increased concentration of MgCl2.
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