Rapid, parallel identification of cell lines
OwnerKurt Jarnigan, Hua Zhouinventors
Life of this patent
Estimating the term…
Abstract
Host cells that exhibit regulated expression of a test gene, are rapidly identifyed by providing a plurality of host cells, each host cell comprising a polynucleotide, the polynucleotide comprising in order a regulatable promoter; a test gene; an IRES sequence; and a surface marker coding sequence, where the surface marker comprises a secretion signal sequence, a detectable label protein, and a membrane anchor; wherein expression of said test gene also results in expression of said surface marker; inducing said promoter; and selecting a host cell that displays said surface marker on its surface.
Claims13
A polynucleotide, comprising:
- a regulatable promoter;
- a test gene;
- an IRES sequence;
- and
- a surface marker coding sequence, said surface marker comprising a secretion signal sequence, a detectable label protein, and a membrane anchor;
- wherein expression of said test gene also results in expression of said surface marker.
A host cell, comprising:
- a polynucleotide, said polynucleotide comprising a regulatable promoter, a test gene, an IRES sequence, a surface marker coding sequence, said surface marker comprising a secretion signal sequence, a detectable label protein, and a membrane anchor;
- wherein expression of said test gene also results in expression of said surface marker.
A method of identifying a host cell that exhibits regulated expression of a test gene, comprising:
- a) providing a plurality of host cells, each host cell comprising a polynucleotide, said polynucleotide comprising in order a regulatable promoter;
- a test gene;
- an IRES sequence;
- and a surface marker coding sequence, said surface marker comprising a secretion signal sequence, a detectable label protein, and a membrane anchor;
- wherein expression of said test gene also results in expression of said surface marker;
- b) inducing said promoter;
- and
- c) selecting a host cell that displays said surface marker on its surface.
A method of identifying a host cell that exhibits regulated expression of a test gene, comprising:
- a) providing a plurality of host cells, each host cell comprising a polynucleotide, said polynucleotide comprising in order a regulatable promoter;
- a test gene;
- an IRES sequence;
- and a surface marker coding sequence, said surface marker comprising a secretion signal sequence, a detectable label protein, and a membrane anchor;
- wherein expression of said test gene also results in expression of said surface marker;
- b) selecting a plurality of host cells that do not exhibit said surface marker in the absence of induction of said promoter;
- c) inducing said promoter;
- and
- d) selecting a host cell that exhibits expression of said surface marker.
Dependencies are inferred from the claim text.