Method of in situ diagnosis by spectroscopic analysis of biological stain compositions

A method for making an in situ analysis of biological tissue and cells of living organisms comprised of:

• a) applying to the tissue or cells a photo-reactive biological stain or dye, or a combination of photo-reactive biological stains or dyes, and
• b) illuminating the stained tissue or cells with light while simultaneously measuring and recording the changes of the reflected light spectrum of the stained tissue or cells and
• c) correlating the change in the reflected light spectrum of the photo-reactive biological stain or dye composition as a cytochemical or histochemical property of a particular type of tissue or cells.

A method for the cytotoxic destruction of dysplasic, pre-cancerous or cancerous cells and tissues by means of

• a) applying to the tissue or cells a biological stain or dye or a combination of biological stains and dyes as a photosensitizer and
• b) irradiating the stained tissue or cells with light of a suitable and sufficient intensity and quality to induce photo oxidation of the biological stain or dye and
• c) simultaneously monitoring the change of the reflected spectrum of the stained or dyed tissue or cells during photo irradiation.