Immunomodulatory formulations and methods for use thereof
AssigneeDynavax Technologies Corporation
InventorsGary Van Nest, Stephen Tuck
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Abstract
The invention provides new compositions and methods for immunomodulation of individuals. Immunomodulation is accomplished by administration of immunomodulatory polynucleotide/microcarrier (IMP/MC) complexes. The IMP/MC complexes may be covalently or non-covalently bound, and feature a polynucleotide comprising at least one immunostimulatory sequence bound to a nonbiodegradable microcarrier or nanocarrier.
Claims66
An immunomodulatory polynucleotide/microcarrier (IMP/MC) complex, comprising:
- a polynucleotide comprising an immunostimulatory sequence (ISS) linked to a nonbiodegradable microcarrier (MC), wherein the ISS comprises the sequence 5′-C, G-3′, with the proviso that if the MC is gold, latex or magnetic, the linkage is other than by biotin/avidin.
A method of modulating an immune response in an individual comprising administering to an individual an immunomodulatory polynucleotide/microcarrier (IMP/MC) complex in an amount sufficient to modulate an immune response in said individual, wherein said MC is a nonbiodegradable MC and wherein the ISS comprises the sequence 5′-C, G-3′.
A method of increasing interferon-gamma (IFN-γ) in an individual, comprising:
- administering an effective amount of an immunomodulatory polynucleotide/microcarrier (IMP/MC) complex to said individual, wherein said MC is a nonbiodegradable MC, wherein the ISS comprises the sequence 5′-C, G-3′ and wherein an effective amount is an amount sufficient to increase IFN-γ in said individual.
A method of increasing interferon-alpha (IFN-α) in an individual, comprising:
- administering an effective amount of an immunomodulatory polynucleotide/microcarrier (IMP/MC) complex to said individual, wherein said MC is a nonbiodegradable MC, wherein the ISS comprises the sequence 5′-C, G-3′ and wherein an effective amount is an amount sufficient to increase IFN-α in said individual.
A method of reducing levels of IgE in an individual, comprising:
- administering an effective amount of an immunomodulatory polynucleotide/microcarrier (IMP/MC) complex to an individual, wherein said MC is a nonbiodegradable MC, wherein the ISS comprises the sequence 5′-C, G-3′ and wherein an effective amount is an amount sufficient to reduce levels of IgE in said individual.
A kit, comprising:
- a container comprising an immunomodulatory polynucleotide/microcarrier (IMP/MC) complex, wherein said MC is a nonbiodegradable MC and wherein the ISS comprises the sequence 5′-C, G-3′;
- and
- instructions for use of IMP/MC complex in immunodulation of an individual.
Dependencies are inferred from the claim text.