L-lysine-producing corynebacteria and process for the preparation of lysine
AssigneeDegussa-Huls AG
InventorsCaroline Kreutzer, Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
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Abstract
The invention relates to L-lysine-producing strains of corynebacteria with enhanced pyc gene (pyruvate carboxylase gene), in which strains additional genes, chosen from the group consisting of the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the lysE gene (lysine export carrier gene) and the dapB gene (dihydrodipicolinate reductase gene), but especially the dapA gene, are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
Claims26
L-Lysine-producing corynebacteria with an enhanced pyc gene, in which additionally genes chosen from the group consisting of the dapA gene, the lysC gene, the lysE gene and the dapB gene, individually or together, are enhanced.
Isolated DNA originating from Corynebacterium and capable of replication in corynebacteria, which contains at least the nucleotide sequence additionally containing the 5′ end upstream from the translation region of the dapB gene, shown in SEQ ID No. 1.
A process for the preparation of L-lysine by the fermentation of corynebacteria with an enhanced pyc gene, wherein said corynebacteria used are those in which nucleotide sequences coding for genes chosen from the group consisting of dapA, lysC, lysE and dapB, individually or together, are enhanced.
Escherichia coli K-12 strain DH5α/pEC7lysEpyc, deposited as DSM12872.
Escherichia coli K-12 strain DH5α/pEC7dapBlysE, deposited as DSM12875.
Escherichia coli K-12 strain DH5α/pEC7dapBpyc, deposited as DSM12873.
Corynebacterium glutamicum strain DSM5715aecD::dapA (MA16), deposited as DSM12867.
Corynebacterium glutamicum strain DSM5715aecD::dapA (MC20), deposited as DSM12868.
Escherichia coli K-12 strain DH5α/pEC7lysEdapBpyc, deposited as DSM12874.
DNA capable of replication, with the nucleotide sequence MC20 shown in SEQ ID No. 5.
DNA capable of replication, with the nucleotide sequence MA16 shown in SEQ ID No. 6.
Dependencies are inferred from the claim text.