Purification of human troponin I
AssigneeDiosynth RTP, Inc.
InventorsGregory Conn, Brian Reardon, Xianfang Zeng, Chenming Zhang
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Abstract
The invention is directed to methods for purifying Troponin I, particularly recombinant Tropnin I produced in a bacterial expression system. Recombinant Tropnin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g., by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrafhionate yielded sulfitolyzed Tropnin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.
Claims20
A method of preparing Troponin I, which method comprises protecting free sulfhydryl groups of Troponin I under reducing conditions.
Troponin I comprising sulfhydryl protecting groups.
A method of purifying Troponin I, which method comprises subjecting Troponin I comprising sulfhydryl protecting groups to chromatography.
Dependencies are inferred from the claim text.